FastQCFastQC Report
Wed 6 Sept 2023
SRR12790138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103846246
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA6015830.579301634071587No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA5234420.5040548119572854No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA4022200.3873226192500016No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA3774460.3634661959759239No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA2117880.2039438190187443No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA1688600.16260578162835082No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATGG1615060.1555241582830062No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAATG1593340.15343260458351088No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAG1445960.13924046902956896No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAA1443210.13897565444975257No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA1434520.13813884037753274No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCG1361340.13109188366809138No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAATCG1330320.12810477520776242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)