FastQCFastQC Report
Wed 6 Sept 2023
SRR12790145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32126629
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA3262541.0155251582729081No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACC2943090.9160905117060367No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA2152070.6698710904278192No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATC1852670.5766773725310551No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTAAAAAAAAAAAAAAAAAAAA1518870.47277602639231153No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAATC1377620.42880938426499715No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTAT1309020.4074563814336076No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA1035290.32225291984415794No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCC994910.30968390739034585No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAT986800.30715952177864664No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAC858260.26714909927213343No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAAATCGTATC827760.257655417255262No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA809840.25207748998502144No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAACC800960.24931342781092905No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCCTATC793380.2469540143785394No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCTTATC783760.2439596136899393No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCGTATC774080.24094653690556828No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAC766770.2386711659041476No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATCC736660.229298878509787No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA727850.22655660511409398No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCGTAT727750.22652547828780917No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCGTATC698600.21745200842578286No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA662870.20633039339421513No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA657880.20477716476260238No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCT649200.20207535624107964No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA644730.200683987106148No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA588420.1831564712251634No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAC580100.18056671927826604No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAAATCCTATCC567790.17673500696260414No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCC555490.17290640732957074No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCG547390.1703851344005No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAT539770.16801327023759635No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATC517830.1611840445507059No Hit
TCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTATC508150.1581709677663349No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAACCTAATC506010.15750485368383965No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATCC494380.1538848037869146No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAACC457110.14228383563056055No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAAATCCTATC453330.14110724159699417No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACCT452300.1407866352862605No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAATCCTATCCC441010.13727241659870384No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAA436920.13599932940365453No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAAA436340.13581879381120254No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAATCCTATCC430500.1340009871561688No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAA430440.1339823110603979No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAAAAAAAAAAAAAAAAAAAA424740.13220808196216294No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAT424250.13205556051336728No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAAA420340.13083850160563065No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCCTATC420010.13073578307889072No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACCC415300.1292697095608755No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAA408400.1271219585472226No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAATCGTATCC406370.12649008397364067No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAA404410.12587999817845813No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAA403850.1257056879512631No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAACCTTATC399420.12432676954684538No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAATCGTATCC396880.12353614815921085No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA396630.12345833109349878No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAAT396600.12344899304561334No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAAAAAA394050.12265525897535033No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAA390950.12169032736052078No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATC378650.11786172772748738No Hit
CCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAAATCCTATCCC367080.1142603539263332No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAAC366540.11409226906439515No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAAA356500.11096713570539878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA356210.1108768679091728No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAACC349810.10888475102694405No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCCT345400.10751205798778328No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAAC344740.10730662093430343No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCGT336680.1047977987357466No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAATCGTATCC334710.10418460025793555No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAA323180.1005956771872953No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACC322090.10025639478079072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)