FastQCFastQC Report
Wed 6 Sept 2023
SRR12790146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36632586
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA3676821.0037020045486278No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA3091660.8439644419315634No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACC2321390.6336953661966426No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA2003830.5470075194800608No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAATC1931980.5273938345493818No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATC1526160.4166126846736946No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAT1493120.40759339239659464No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAA1303910.35594265717413454No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAACC1151830.31442770652336693No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA1106050.3019306362919615No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTAT1062160.2899495001526783No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAA973290.2656896785828879No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA959090.26181334836694303No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA952620.260047161289678No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAC911330.24877577575331428No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAAA855680.23358438304082602No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATC781440.21331827351746338No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA763440.20840461549725153No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCC763320.2083718577771168No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA762610.20817804126631953No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAAAAAAAAAAAAAAAAAAA745640.20354555367726426No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAA723520.19750721393242618No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAAA719880.19651356308833887No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAC714100.19493573290184862No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGAAAAAAAAAAAAAAAAAA713690.19482381069138827No Hit
GTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAAAAA708770.1934807441658637No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATCC689520.18822585989424825No Hit
CCCCGCCCCTTGCCTCTCGGAAAAAAAAAAAAAAAAAAAATCCTATCCC663080.1810082422245593No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA645780.17628567090513347No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATCC638350.17425742206679048No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAAAAAAAAAAAAAAA636300.17369781101448858No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAA616600.16832008529236783No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAC608830.16619902291364308No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCT604030.16488871410825323No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAAA600440.1639087123142221No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAAAAAAAAAAAAAAAAA600290.16386776516405369No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA588330.16060291239062402No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCGTATC577990.15778028883901343No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCGTAT574310.15677571875488122No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAAATCCTATCC562240.15348083807132806No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAA558130.15235888615671303No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA550530.15028423054817913No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAAC503110.13733947147493217No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA499270.13629122443062033No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAACC498370.1360455415296097No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA485050.13240943459465299No Hit
TGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAAT473740.1293220194719532No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAT467520.12762407764496886No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAAAA462410.12622914472923097No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCC439750.1200433952437865No Hit
CCCCGCCCCTTGCCTCTCGGAAAAAAAAAAAAAAAAAAATCCTATCCCC430550.117531970033456No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTAAAAAAAAAAAAAAAAAAAA428630.11700784651130008No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA421230.11498778710299076No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAATCCTATCCC411340.11228800500188549No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAA393600.10744532204196558No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCG383580.10471005241071434No Hit
TCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTATC383570.10470732260070309No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCCTATC382800.10449712722983848No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA382190.10433060881915351No Hit
TGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAAAAAAAATCCT379410.10357172163603193No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAACC377410.10302575963378616No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAA374910.10234330713097897No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGAAAAAAAAAAAAAAAAA372780.10176185759858722No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAA371660.1014561188773296No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA369300.1008118837146796No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAACCC368450.10057984986372515No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACCT366380.1000147791914008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)