FastQCFastQC Report
Wed 6 Sept 2023
SRR12790149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31093218
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA3094770.9953199440469622No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACC2440560.7849171481703823No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATC2158130.694083835259509No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA2031260.653280725076446No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAATC1454430.4677643851466259No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTAAAAAAAAAAAAAAAAAAAA1432140.46059561927620357No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTAT1249410.4018271765887983No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCTTATC1193240.38376214388616836No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAT1047030.3367390277841297No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA1013720.32602608067135413No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATCC831290.26735412204680775No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCC771240.24804122879786838No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAAATCGTATC751530.24170222586803333No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA744210.2393480147342742No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAC717470.23074806859811037No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCGTATC714820.22989579270952268No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA709430.22816229571348967No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCT668950.21514337949838452No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCGTAT656560.21115858770230858No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAACC649250.20880759270397808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA642800.20673318535251No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAC638770.205437082774771No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCCTATC625730.20124324217583398No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA622440.20018513361981383No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA619490.19923637366836716No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAT604630.19445719642141898No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCC592360.19051099825048665No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAAATCCTATCC565400.1818402971348929No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA562800.1810041019234484No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATC557680.17935744058398845No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCG545590.1754691328507715No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATCC527580.169676872943804No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAT487860.15690238302127493No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAACCTAATC476960.15339679540406528No Hit
TCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTATC470690.15138027849031258No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAC469810.1510972585725929No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACCT451590.14523745982162412No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAATCCTATCCC445870.1433978303564462No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAATCGTATC429530.13814266506606038No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATC421010.13540251768086534No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAAT420550.13525457545114822No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAAA414740.13338600076711263No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAA411100.13221532747109033No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAATCCTATCC409700.13176506851108175No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAAAAAAAAAAAAAAAAAAAA408530.1313887806659317No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAA406310.13067479860077524No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAAA400860.12892200479217045No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAA397670.1278960575904366No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA388210.12485359347495008No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAA387870.12474424487037657No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAAATCCTATC380790.1224672209869046No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAAATCTTATC379780.12214239130861271No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAAAAAAAAAAA379560.12207163632918278No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAACC378160.12162137736917421No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAA377110.12128368314916776No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAA375970.12091704371030364No Hit
CCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAAATCCTATCCC374300.12037994909372199No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCCT363130.11678752581993924No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCCTATC349000.11224312645928124No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA346040.11129115037240597No Hit
TAATCAAACTCCGAATACCATTAAAAAAAAAAAAAAAAAAAACCTTATC345670.11117215336154657No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAAA345520.11112391133011708No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAATCTTATCC344250.11071546213068072No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTAAAAAAAAAAAAAAAAAAAT335970.10805250199577283No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCGT325090.10455334664942045No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAATC318060.10229240344309168No Hit
ATCTTGAGTTGTGATGCATAGTAAAAAAAAAAAAAAAAAAATCGTATCC312710.1005717709887732No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCTTAT311400.1001504572476223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)