FastQCFastQC Report
Wed 6 Sept 2023
SRR12790150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12790150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35284099
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA3485600.9878670842636508No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA2931850.8309267015717193No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAATC2057970.5832570643223737No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA1894300.5368707303536361No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACC1890510.5357965921136316No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATC1783650.505510995193614No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAT1578170.44727513093079124No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAA1263950.35822085183470326No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA1021340.2894618337852413No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTAT1017170.2882799983074529No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAA947500.2685345600010929No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAACC915290.25940580202997393No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAA912290.25855556067904695No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAAAAAAAAAAAAAAAAAAA902720.25584329076959No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATC835860.23689424519526486No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCAAAAAAAAAAAAAAAAAAAA808030.22900683959649926No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA741210.2100691305735198No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAA733760.20795769788538457No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAC725210.20553451003524278No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAATCC724120.20522558901107266No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAAAAAAAAAAAAAAAAAAA720430.20417979214943255No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAATCC703360.1993419188626582No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAA690930.19581908553198427No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAAAAAAA689970.19554700829968763No Hit
CCCCGCCCCTTGCCTCTCGGAAAAAAAAAAAAAAAAAAAATCCTATCCC689500.19541380382137574No Hit
GTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAAAAA685630.19431699247867998No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGAAAAAAAAAAAAAAAAAA684970.19412993938147605No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAAAA635280.18004710847228947No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCT619170.17548131241781176No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAAAAAAAAAAAAAAA617610.17503918691532977No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAC589960.16720279579761976No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAAA580790.16460389140161974No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAAAAAAAAAAAAAAAAA578740.164022893145153No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA573090.16242160526757393No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAAATCCTATCC564140.1598850519039752No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAA559220.15849065608845503No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAACCC557000.1578614774887691No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAA542980.15388801624210385No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAATCGTATC541960.1535989341827887No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAT529940.15019230050340807No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAAAAAAA520890.14762740576144512No Hit
ATCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCGTAT517610.14669780855109835No Hit
TGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAAT514220.1457370358245509No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAAAAC492870.13968615154378747No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA483920.13714959818018876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA466380.13217852041510258No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCC459950.13035616978628248No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGAAAAAAAAAAAAAAAAAAA445100.12614747509919413No Hit
CCCCGCCCCTTGCCTCTCGGAAAAAAAAAAAAAAAAAAATCCTATCCCC437570.1240133693083675No Hit
CCCCGCCCCTTGCCTCTCGGCAAAAAAAAAAAAAAAAAAATCCTATCCC418180.11851797604354301No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAAAAC410330.11629317784195084No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAAAA405730.11498947443719619No Hit
ATCGCATGATGAGTAATAGAAAGGAGCTTAAAAAAAAAAAAAAAAAAAA405240.11485060168321146No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAAAAACC404200.11455585134822346No Hit
TGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAAAAAAAATCCT384200.10888757567537716No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAATCG381630.10815920225141643No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAAAAAAAAAAAAAAAAAAAA371270.10522303545288204No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAA367820.10424525789931606No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAAAAACCT364470.1032958217241143No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAA364010.10316545138363885No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCAAAAAAAAAAAAAAAAAAAATC363230.10294438863239783No Hit
TCTTGAGTTGTGATGCATAGTGAAAAAAAAAAAAAAAAAAAATCCTATC359370.1018504114275385No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA358320.10155282695471408No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAAAAAAAAAAAAAAAAAAA355980.10088963870099107No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGAAAAAAAAAAAAAAAAA354970.10060339077951233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)