FastQCFastQC Report
Wed 6 Sept 2023
SRR12874340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12874340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84109
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length22-35
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGAATGCGCAGGCTGAAGCGCAAAAG13391.5919818330975282No Hit
CGAATGCGCAGGCTGAAGCGCAAAAGAAG9971.1853666076163074No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGC8681.0319941980049696No Hit
TATCCGCGCAAGATGTTGACGTTGCAGA6430.7644841812410087No Hit
CCAATGGAAAAAGCATGACATTTAGAAGT5100.6063560379983117No Hit
TATGCTGATGGACCAGGAAGCCCTGAATC4790.5694991023552771No Hit
ACTGAATCTACAGGTGAACTTCTAGATC4720.5611765685003983No Hit
ACTGAATCTACAGGTGAACTTCTAGATCC4410.5243196328573636No Hit
TCTGTAAAGAAATATATACTTGAGTGGTG4190.49816309788488744No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTTG3810.4529836283869741No Hit
AGTTACTTACCACAGACTGTACGGCAAG3740.4446610945320953No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGAG3490.4149377593360996No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGA3440.40899309229690045No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAAA3330.39591482481066237No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAA3310.39353695799498273No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGA3240.3852144241401039No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTG2840.3376570878265108No Hit
ACTGTACAACTCGTGTGGAAGACATTGC2690.3198230867089134No Hit
ACTGTACAACTCGTGTGGAAGACATTGCC2610.31031161944619484No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAG2560.3043669524069957No Hit
ACATGGACCAACCCAAGTATTAAGAGTG2480.29485548514427706No Hit
AAGACATTGCCTCCTTTTGAAGCCAATGG2440.2900997515129178No Hit
CTTTGGGTGGAATGGACTACTCCAAGGG2390.2841550844737186No Hit
GCTTTTGGTCCAGGTACTGAGGGACAAG2300.2734546838031602No Hit
AAAAAGCCTTTTCCAGAAGATCTGAAAT2160.25680961609340264No Hit
TCCAAAGGACCTACTGTTCGGACAAAAA2150.2556206826855628No Hit
AAAGGACCTACTGTTCGGACAAAAAAAG2090.2484870822385238No Hit
TGGAGCCAGATTCCTCCTGAAGACACAGC2040.24254241519932468No Hit
TGGCCTAATGTTCCAGATCCTTCAAAG2020.24016454838364504No Hit
GAGGAAGATTTTGTTAGACTTAAACAGC1980.2354088147522857No Hit
CCAATGGAAAAAGCATGACATTTAGAAG1940.23065308112092642No Hit
AGTTACTTACCACAGACTGTACGGCAAGG1930.2294641477130866No Hit
CTGTAAAGAAATATATACTTGAGTGGTG1880.22351948067388747No Hit
TCCAAAGGACCTACTGTTCGGACAAAAAA1850.21995268045036798No Hit
TCAGATAAAGCACCCTGTATCACAGACTG1730.20568547955629007No Hit
AAGCGAATGCGCAGGCTGAAGCGCAAAAGA1730.20568547955629007No Hit
GAGGAAGATTTTGTTAGACTTAAACAGCA1720.20449654614845023No Hit
ATGCTTTGGGTGGAATGGACTACTCCAAG1710.20330761274061038No Hit
TTAATAAGCGAGACCTAATTAAAAA1690.20092974592493076No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGGG1690.20092974592493076No Hit
ACCATCATTGGAAATGAAACTGCTGTGAA1660.19736294570141125No Hit
TGGGTGGAATGGACTACTCCAAGGGAAT1620.19260721207005194No Hit
TCAGATGGCAATTTCACTGATGTAAGTG1590.18904041184653247No Hit
ATCTTGGATTATGAAGTGACTCTCACAAG1590.18904041184653247No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGAG1580.18785147843869265No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTT1560.18547361162301298No Hit
ACATTGCCTCCTTTTGAAGCCAATGGAAA1560.18547361162301298No Hit
TAAAATAGATCCATCCCATACTCAAGG1540.18309574480733334No Hit
ATGTCATCTTCTAGGCCAAGCATTTCTAG1540.18309574480733334No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTGA1520.1807178779916537No Hit
TACAGAACTGTACAACTCGTGTGGAAGA1520.1807178779916537No Hit
ACAGATATAGCTTCATTAAATATTCAGCT1510.17952894458381385No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGCG1510.17952894458381385No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAAT1470.17477321095245454No Hit
AATGAGGAAGATTTTGTTAGACTTAAACA1440.17120641072893508No Hit
TATGAAGTGACTCTCACAAGATGGAAAT1430.17001747732109526No Hit
AAGCGAGACCTAATTAAAAAAC1430.17001747732109526No Hit
TAATAAGCGAGACCTAATTAAAAA1410.1676396105054156No Hit
AAGCGAGACCTAATTAAAAAACAC1370.16288387687405628No Hit
TCTACTGTGTATTTTGTCAACATTGAAGT1330.15812814324269697No Hit
ACAGTTAATGCCACAAAACTGACAGTAAA1310.15575027642701733No Hit
AAAGGACCTACTGTTCGGACAAAAAAAGT1300.15456134301917748No Hit
TTACTTACCACAGACTGTACGGCAAG1290.1533724096113377No Hit
ACAGCATCCACCCGATCTTCATTCACTG1280.15218347620349784No Hit
TCTACAGGTGAACTTCTAGATCCATGTGG1270.150994542795658No Hit
TCAGTCAATGAGGAAGATTTTGTTAGACT1270.150994542795658No Hit
TGGACTACTCCAAGGGAATCTGTAAAGA1250.14861667597997835No Hit
ACTGCAGTTTGTGTGCTAAAGGA1220.1450498757564589No Hit
CGCAAGATGTTGACGTTGCAGACTTGGCT1210.14386094234861904No Hit
AGTGTCACCTTTACAGATATAGCTTCATT1200.14267200894077922No Hit
CTGTCTAGTATCTTAAAATTGACATGGAC1170.13910520871725976No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAA1160.1379162753094199No Hit
AGTCCAGATATTTCACATTTTGAAAGGTC1140.13553840849374027No Hit
AAACAGCAGATTTCAGATCATATTTCACA1140.13553840849374027No Hit
ACTGCAGTTTGTGTGCTAAAGG1110.13197160827022078No Hit
GAGACAAACTTCACTTTAAAATCTGAATG1100.13078267486238096No Hit
GATAAAGCACCCTGTATCACAGACTGGC1100.13078267486238096No Hit
CCTACTGTTCGGACAAAAAAAGTAGGGA1090.12959374145454114No Hit
AAACAGCAGATTTCAGATCATATTTCAC1080.12840480804670132No Hit
TATCCGCGCAAGATGTTGACGTTGCAG1070.12721587463886147No Hit
TCATCAGTCAATGAGGAAGATTTTGTTAG1070.12721587463886147No Hit
GAGTGGGACCAACTTCCTGTTGATGTTCA1060.12602694123102165No Hit
ACAGGTGAACTTCTAGATCCATGTGGTT1060.12602694123102165No Hit
ACTCCAGTATATGCTGATGGACCAGGAAG1050.12483800782318183No Hit
ATCACCTATGAAGATAGACCATCTAAAGC1050.12483800782318183No Hit
ACTGCTGTGAATGTGGATTCTTCCCACAC1050.12483800782318183No Hit
GATTATGAAGTGACTCTCACAAGATGGA1040.123649074415342No Hit
GAGTGAAACCCAATGGAAAAAGCATGAC1040.123649074415342No Hit
AAAAACGAAGCTGTCTTAGAGTGGGACC1030.12246014100750217No Hit
ACAGGTGAACTTCTAGATCCATGTGGTTA1030.12246014100750217No Hit
AATGGAAAAATCTTGGATTATGAAGTGAC1020.12127120759966234No Hit
ATCACCTATGAAGATAGACCATCTAAAG1020.12127120759966234No Hit
TATCCGCGCAAGATGTTGACGTTGCAGAC1010.12008227419182252No Hit
ATTATGAAGTGACTCTCACAAGATGGA1010.12008227419182252No Hit
AGTGACACATTGTACATGGTACGAATGG990.11770440737614285No Hit
TCAGATGGCAATTTCACTGATGTAAGTGT990.11770440737614285No Hit
TGTGTGCTAAAGGAAAAATGTATGGATT970.11532654056046321No Hit
GAAGTTTCTGCAGCAGATGCTTTTGGTCC960.11413760715262339No Hit
AAAGGACCTACTGTTCGGACAAAAA960.11413760715262339No Hit
AGCACTGTCCAGTATTCTACCGTGGTAC960.11413760715262339No Hit
ATGCTTTGGGTGGAATGGACTACTCCAA930.1105708069291039No Hit
TACAGACACCAAGTTCCGTCAGTCCAAGT930.1105708069291039No Hit
TGGACCAACCCAAGTATTAAGAGTGTTAT930.1105708069291039No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGG920.10938187352126406No Hit
ATGAAGTGACTCTCACAAGATGGA910.10819294011342424No Hit
GAGTGGGACCAACTTCCTGTTGATGTTC910.10819294011342424No Hit
AAGAAGATGGTACCGTGCATCGCAC910.10819294011342424No Hit
AAAGGACCTACTGTTCGGACAAAAAA900.10700400670558441No Hit
AGAAATCTTGTTGGCAAATCAGATGCAGC900.10700400670558441No Hit
CCAAAGTTTGCTCAAGGAGAAATTGAAGC890.1058150732977446No Hit
TCCGAGTCTACCCAGCCCTTGTTAGATTC890.1058150732977446No Hit
ACAAAACTGACAGTAAATCTCACAAATG880.10462613988990477No Hit
AAAGGACCTACTGTTCGGACAAA880.10462613988990477No Hit
TCATTGGACCTGTTCAAAAAGGAAAAAA880.10462613988990477No Hit
ACAGCATCCACCCGATCTTCATTCACTGT870.10343720648206495No Hit
AGCCAGATTCCTCCTGAAGACACAGCAT870.10343720648206495No Hit
AGTTACTTACCACAGACTGTACGGCAA860.10224827307422511No Hit
TTGACGTTGCAGACTTGGCTAGTGCAAG860.10224827307422511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)