FastQCFastQC Report
Wed 6 Sept 2023
SRR12874341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12874341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218926
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length21-45
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGAATGCGCAGGCTGAAGCGCAAAAG27891.2739464476581128No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGC22711.0373368170066597No Hit
TATCCGCGCAAGATGTTGACGTTGCAGA19320.8824899737810951No Hit
CGAATGCGCAGGCTGAAGCGCAAAAGAAG17310.7906781286827513No Hit
CCAATGGAAAAAGCATGACATTTAGAAGT16120.7363218621817418No Hit
ACTGAATCTACAGGTGAACTTCTAGATC12610.5759937147712012No Hit
TATGCTGATGGACCAGGAAGCCCTGAATC12560.5737098380274613No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAAA11780.5380813608251189No Hit
TCTGTAAAGAAATATATACTTGAGTGGTG11650.5321432812913952No Hit
ACTGAATCTACAGGTGAACTTCTAGATCC11580.5289458538501595No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGA11420.5216374482701918No Hit
AGTTACTTACCACAGACTGTACGGCAAG11090.5065638617615085No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTTG10530.4809844422316216No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTG9690.44261531293679146No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGAG9050.41338169061692076No Hit
GCTTTTGGTCCAGGTACTGAGGGACAAG8500.38825904643578196No Hit
CTTTGGGTGGAATGGACTACTCCAAGGG7920.3617660762083992No Hit
CCAATGGAAAAAGCATGACATTTAGAAG7520.34349506225848003No Hit
ACATGGACCAACCCAAGTATTAAGAGTG7250.3311621278422846No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAG7200.32887825109854474No Hit
ACTGTACAACTCGTGTGGAAGACATTGC7050.32202662086732503No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGA7040.32156984551857704No Hit
TCCAAAGGACCTACTGTTCGGACAAAAA6950.31745886737984524No Hit
TCCAAAGGACCTACTGTTCGGACAAAAAA6630.30284205621990995No Hit
ACTGTACAACTCGTGTGGAAGACATTGCC6340.28959557110621853No Hit
AAAGGACCTACTGTTCGGACAAAAAAAG6310.2882252450599746No Hit
CTGTAAAGAAATATATACTTGAGTGGTG6170.2818303901775029No Hit
TGGAGCCAGATTCCTCCTGAAGACACAGC5960.2722381078537953No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAA5910.26995423111005545No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGGG5350.24437481158016863No Hit
TTAATAAGCGAGACCTAATTAAAAA5240.23935028274394088No Hit
TAAAATAGATCCATCCCATACTCAAGG5010.22884444972273735No Hit
TCAGATGGCAATTTCACTGATGTAAGTG4890.2233631455377616No Hit
AAAAAGCCTTTTCCAGAAGATCTGAAAT4870.22244959484026566No Hit
AGTTACTTACCACAGACTGTACGGCAAGG4870.22244959484026566No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTGA4720.21559796460904598No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTT4630.21148698647031416No Hit
AAGACATTGCCTCCTTTTGAAGCCAATGG4610.21057343577281823No Hit
GATAAAGCACCCTGTATCACAGACTGGC4530.2069192329828344No Hit
TACAGAACTGTACAACTCGTGTGGAAGA4480.20463535623909448No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAAT4340.1982405013566228No Hit
TGGGTGGAATGGACTACTCCAAGGGAAT4200.1918456464741511No Hit
GAGGAAGATTTTGTTAGACTTAAACAGC4160.19001854507915916No Hit
AGTCCAGATATTTCACATTTTGAAAGGTC4160.19001854507915916No Hit
TATGAAGTGACTCTCACAAGATGGAAAT4010.18316691484793948No Hit
ATCTTGGATTATGAAGTGACTCTCACAAG3990.18225336415044355No Hit
TCAGATAAAGCACCCTGTATCACAGACTG3970.18133981345294758No Hit
ACTGCTGTGAATGTGGATTCTTCCCACAC3970.18133981345294758No Hit
AAGCGAATGCGCAGGCTGAAGCGCAAAAGA3960.1808830381041996No Hit
AAGCGAGACCTAATTAAAAAACACATCT3940.17996948740670363No Hit
TGGCCTAATGTTCCAGATCCTTCAAAG3850.17585850926797184No Hit
CCTACTGTTCGGACAAAAAAAGTAGGGA3840.17540173391922384No Hit
TAATAAGCGAGACCTAATTAAAAA3820.17448818322172788No Hit
ACAGATATAGCTTCATTAAATATTCAGCT3800.17357463252423191No Hit
TCATTGGACCTGTTCAAAAAGGAAAAAA3790.17311785717548395No Hit
AGAAATCTTGTTGGCAAATCAGATGCAGC3710.16946365438550012No Hit
GAGGAAGATTTTGTTAGACTTAAACAGCA3700.16900687903675216No Hit
TATCCGCGCAAGATGTTGACGTTGCAG3630.1658094515955163No Hit
ATGCTTTGGGTGGAATGGACTACTCCAAG3620.1653526762467683No Hit
ATTATGAAGTGACTCTCACAAGATGGA3540.16169847345678448No Hit
AAAAACGAAGCTGTCTTAGAGTGGGACC3500.15987137206179255No Hit
ACCATCATTGGAAATGAAACTGCTGTGAA3470.15850104601554865No Hit
CCAAAGTTTGCTCAAGGAGAAATTGAAG3460.15804427066680066No Hit
AGTGGCTACAGACACCAAGTTCCGTCAG3400.15530361857431277No Hit
CGCAAGATGTTGACGTTGCAGACTTGGCT3380.1543900678768168No Hit
ACAGCATCCACCCGATCTTCATTCACTG3340.1525629664818249No Hit
GAGTGGGACCAACTTCCTGTTGATGTTC3340.1525629664818249No Hit
GATTATGAAGTGACTCTCACAAGATGGA3320.15164941578432897No Hit
AATGAGGAAGATTTTGTTAGACTTAAACA3320.15164941578432897No Hit
TCAGTCAATGAGGAAGATTTTGTTAGACT3310.15119264043558098No Hit
TTACTTACCACAGACTGTACGGCAAG3300.150735865086833No Hit
TGTAAAGAAATATATACTTGAGTGGTG3270.14936553904058905No Hit
ATCACCTATGAAGATAGACCATCTAAAGC3260.14890876369184108No Hit
ACATTGCCTCCTTTTGAAGCCAATGGAAA3230.14753843764559713No Hit
TGGACTACTCCAAGGGAATCTGTAAAGA3220.14708166229684916No Hit
TGTGTGCTAAAGGAAAAATGTATGGATT3220.14708166229684916No Hit
TTGACGTTGCAGACTTGGCTAGTGCAAG3200.14616811159935322No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAG3190.14571133625060523No Hit
AGTGACACATTGTACATGGTACGAATGG3130.14297068415811734No Hit
AAGAAGATGGTACCGTGCATCGCAC3110.1420571334606214No Hit
ATCACCTATGAAGATAGACCATCTAAAG3090.14114358276312544No Hit
CAAGGCTACAGAACTGTACAACTCGTGTG3090.14114358276312544No Hit
GAGTGGGACCAACTTCCTGTTGATGTTCA3070.14023003206562948No Hit
ACAGGTGAACTTCTAGATCCATGTGGTTA3060.1397732567168815No Hit
AAAAAGGAAAAAATTAATACTGAAGGAC3050.13931648136813354No Hit
TATCCGCGCAAGATGTTGACGTTGCAGAC3030.13840293067063758No Hit
GTGCTGTTCTGCTTTAATAAGCGAGACC2950.13474872788065373No Hit
GAGACAAACTTCACTTTAAAATCTGAATG2900.13246485113691384No Hit
AAAAAGCCTTTTCCAGAAGATCTGAAA2880.1315513004394179No Hit
AAGCGAGACCTAATTAAAAAAC2870.1310945250906699No Hit
GAGTGAAACCCAATGGAAAAAGCATGAC2850.13018097439317394No Hit
CAACTCAAGGGCGAGTGGAACCGTAAAAG2830.12926742369567798No Hit
ATGTCATCTTCTAGGCCAAGCATTTCTAG2830.12926742369567798No Hit
AAACAGCAGATTTCAGATCATATTTCAC2800.12789709764943405No Hit
ACAGGTGAACTTCTAGATCCATGTGGTT2780.12698354695193811No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGG2780.12698354695193811No Hit
TCTACTGTGTATTTTGTCAACATTGAAGT2770.12652677160319012No Hit
ATTGAAGTCTGGGTAGAAGCAGAGAATG2770.12652677160319012No Hit
AAGCGAGACCTAATTAAAAAACAC2740.1251564455569462No Hit
AAAGGACCTACTGTTCGGACAAAAAAAGT2710.12378611951070223No Hit
AAGCGAGACCTAATTAAAAAACACATCTG2680.12241579346445831No Hit
AAACAGCAGATTTCAGATCATATTTCACA2670.12195901811571035No Hit
CTGTCTAGTATCTTAAAATTGACATGGAC2640.12058869206946639No Hit
GAAGCCATAGTCGTGCCTGTTTGCTTAGC2600.11876159067447448No Hit
TCTACAGGTGAACTTCTAGATCCATGTGG2590.11830481532572651No Hit
ACTTGGAGCCAGATTCCTCCTGAAGACAC2590.11830481532572651No Hit
TTACTTACCACAGACTGTACGGCAAGG2580.11784803997697851No Hit
AGCACTGTCCAGTATTCTACCGTGGTAC2580.11784803997697851No Hit
TACAGAACTGTACAACTCGTGTGGAAGAC2520.11510738788449064No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAA2520.11510738788449064No Hit
ACTCAAGGCTACAGAACTGTACAACTCG2520.11510738788449064No Hit
TACAGACACCAAGTTCCGTCAGTCCAAGT2510.11465061253574267No Hit
AATGGAAAAAGCATGACATTTAGAAGT2510.11465061253574267No Hit
AGTTACTTACCACAGACTGTACGGCAA2470.11282351114075076No Hit
AGATTTCAGATCATATTTCACAATCCTG2470.11282351114075076No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGCG2470.11282351114075076No Hit
TACAGACACCAAGTTCCGTCAGTCCAAG2440.11145318509450683No Hit
AATGATCGCTATCTAGCAACCCTAACAG2430.11099640974575883No Hit
CGAGACCTAATTAAAAAACACATCTGGCC2420.11053963439701087No Hit
TCCGAGTCTACCCAGCCCTTGTTAGATTC2360.10779898230452299No Hit
ACAAAACTGACAGTAAATCTCACAAATG2340.10688543160702704No Hit
AGTGTTGTGGAAATAGAAGCAAATGACA2320.10597188090953108No Hit
GTTACTTACCACAGACTGTACGGCAAG2310.10551510556078308No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGAG2300.10505833021203512No Hit
GTAAATCTCACAAATGATCGCTATCTAGC2280.10414477951453917No Hit
AACGAAGCTGTCTTAGAGTGGGACCAACT2260.1032312288170432No Hit
GATAAAGCACCCTGTATCACAGACTGGCA2240.10231767811954724No Hit
GGCTACATGCCTCAGTGAAGGACTAGTAG2200.10049057672455533No Hit
ACAGTTAATGCCACAAAACTGACAGTAAA2190.10003380137580736No Hit
AAAGGACCTACTGTTCGGACAAAAAA2190.10003380137580736No Hit
AGTGTCACCTTTACAGATATAGCTTCATT2190.10003380137580736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)