FastQCFastQC Report
Wed 6 Sept 2023
SRR12874342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12874342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111312
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length20-35
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGAATGCGCAGGCTGAAGCGCAAAAG17131.5389176369124622No Hit
CGAATGCGCAGGCTGAAGCGCAAAAGAAG13731.2334698864453069No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGC11661.0475061089550093No Hit
TATCCGCGCAAGATGTTGACGTTGCAGA8500.7636193761678884No Hit
CCAATGGAAAAAGCATGACATTTAGAAGT6770.6082003737243065No Hit
ACTGAATCTACAGGTGAACTTCTAGATCC6340.5695702170475779No Hit
TATGCTGATGGACCAGGAAGCCCTGAATC6130.5507043265775478No Hit
ACTGAATCTACAGGTGAACTTCTAGATC6120.5498059508408797No Hit
TCTGTAAAGAAATATATACTTGAGTGGTG5610.5039887882708064No Hit
AGTTACTTACCACAGACTGTACGGCAAG4960.44559436538737957No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTTG4940.44379761391404343No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAAA4670.41954146902400463No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGAG4610.41415121460399595No Hit
TCAGGCTTGCCTCCAGAAAAACCTAAAA4190.3764194336639356No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGA4050.3638421733505821No Hit
TGGCCTAATGTTCCAGATCCTTCAAAGA4040.36294379761391404No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAG3900.3503665373005606No Hit
ACTGTACAACTCGTGTGGAAGACATTGC3600.3234152652005175No Hit
ACTGTACAACTCGTGTGGAAGACATTGCC3300.29646399310047433No Hit
ACATGGACCAACCCAAGTATTAAGAGTG3180.2856834842604571No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTG3070.27580135115710797No Hit
AAGACATTGCCTCCTTTTGAAGCCAATGG3030.27220784821043553No Hit
AAAAAGCCTTTTCCAGAAGATCTGAAAT2810.25244358200373723No Hit
AAAGGACCTACTGTTCGGACAAAAAAAG2790.25064683053040104No Hit
GCTTTTGGTCCAGGTACTGAGGGACAAG2780.24974845479373292No Hit
TGGAGCCAGATTCCTCCTGAAGACACAGC2690.24166307316372No Hit
TCCAAAGGACCTACTGTTCGGACAAAAAA2630.23627281874371137No Hit
AGTTACTTACCACAGACTGTACGGCAAGG2630.23627281874371137No Hit
CTTTGGGTGGAATGGACTACTCCAAGGG2610.23447606727037518No Hit
TGGCCTAATGTTCCAGATCCTTCAAAG2590.23267931579703896No Hit
CCAATGGAAAAAGCATGACATTTAGAAG2490.2236955584303579No Hit
TTAATAAGCGAGACCTAATTAAAAA2400.21561017680034494No Hit
CTGTAAAGAAATATATACTTGAGTGGTG2390.21471180106367688No Hit
ACATTGCCTCCTTTTGAAGCCAATGGAAA2360.21201667385367254No Hit
GAGGAAGATTTTGTTAGACTTAAACAGC2350.21111829811700447No Hit
TCCAAAGGACCTACTGTTCGGACAAAAA2270.20393129222365963No Hit
TACAGAACTGTACAACTCGTGTGGAAGA2160.19404915912031048No Hit
AGGCAGAGGTCCAAGTAAACCGCTAGCTT2120.19045565617363808No Hit
TTACTTACCACAGACTGTACGGCAAG2120.19045565617363808No Hit
AAGCGAATGCGCAGGCTGAAGCGCAAAAGA2090.18776052896363377No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGGG2070.18596377749029755No Hit
TCAGATGGCAATTTCACTGATGTAAGTG2030.18237027454362514No Hit
ATCTTGGATTATGAAGTGACTCTCACAAG2000.17967514733362083No Hit
ACCATCATTGGAAATGAAACTGCTGTGAA1950.1751832686502803No Hit
TGGGTGGAATGGACTACTCCAAGGGAAT1910.17158976570360787No Hit
AGGAAGAAGCGAATGCGCAGGCTGAAGCG1890.16979301423027168No Hit
AAGCGCAAAAGAAGAAAGATGAGGCAGAG1860.16709788702026737No Hit
GAGGAAGATTTTGTTAGACTTAAACAGCA1860.16709788702026737No Hit
TATGAAGTGACTCTCACAAGATGGAAAT1830.16440275981026306No Hit
AAGTTTGCTGATTGCAAAGCAAAACGTGA1830.16440275981026306No Hit
ACAGATATAGCTTCATTAAATATTCAGCT1820.16350438407359494No Hit
TAATAAGCGAGACCTAATTAAAAA1810.16260600833692684No Hit
ATGCTTTGGGTGGAATGGACTACTCCAAG1780.15991088112692253No Hit
TAAAATAGATCCATCCCATACTCAAGG1770.15901250539025444No Hit
AAGCGAGACCTAATTAAAAAAC1770.15901250539025444No Hit
AGTGTCACCTTTACAGATATAGCTTCATT1720.1545206267069139No Hit
TGGACTACTCCAAGGGAATCTGTAAAGA1720.1545206267069139No Hit
AAGCGAGACCTAATTAAAAAACAC1710.15362225097024582No Hit
TCAGATAAAGCACCCTGTATCACAGACTG1660.1491303722869053No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAAT1660.1491303722869053No Hit
AATGAGGAAGATTTTGTTAGACTTAAACA1650.14823199655023717No Hit
ACAGTTAATGCCACAAAACTGACAGTAAA1630.14643524507690098No Hit
CGCAAGATGTTGACGTTGCAGACTTGGCT1590.14284174213022854No Hit
ACTGCAGTTTGTGTGCTAAAGGA1570.14104499065689236No Hit
AGTGACACATTGTACATGGTACGAATGG1570.14104499065689236No Hit
ATGTCATCTTCTAGGCCAAGCATTTCTAG1550.13924823918355614No Hit
TCTACAGGTGAACTTCTAGATCCATGTGG1540.13834986344688804No Hit
ACAGCATCCACCCGATCTTCATTCACTG1460.1311628575535432No Hit
GATAAAGCACCCTGTATCACAGACTGGC1460.1311628575535432No Hit
GGCTACATGCCTCAGTGAAGGACTAGTAG1430.1284677303435389No Hit
ACTGCAGTTTGTGTGCTAAAGG1430.1284677303435389No Hit
TCAGTCAATGAGGAAGATTTTGTTAGACT1430.1284677303435389No Hit
GAAGTTTCTGCAGCAGATGCTTTTGGTCC1420.1275693546068708No Hit
AAAGGACCTACTGTTCGGACAAAAAA1420.1275693546068708No Hit
AAACAGCAGATTTCAGATCATATTTCAC1410.12667097887020268No Hit
AAACAGCAGATTTCAGATCATATTTCACA1410.12667097887020268No Hit
ATGCTTTGGGTGGAATGGACTACTCCAA1400.12577260313353458No Hit
ATGAAGTGACTCTCACAAGATGGA1380.12397585166019835No Hit
GAGACAAACTTCACTTTAAAATCTGAATG1380.12397585166019835No Hit
ACAGGTGAACTTCTAGATCCATGTGGTTA1370.12307747592353024No Hit
GAATGCGCAGGCTGAAGCGCAAAAGAAG1370.12307747592353024No Hit
AATGGAAAAATCTTGGATTATGAAGTGAC1360.12217910018686215No Hit
TCTACTGTGTATTTTGTCAACATTGAAGT1360.12217910018686215No Hit
AAAGGACCTACTGTTCGGACAAAAAAAGT1360.12217910018686215No Hit
GTGTGGAAGACATTGCCTCCTTTTGAAGC1350.12128072445019404No Hit
TGTGTGCTAAAGGAAAAATGTATGGATT1350.12128072445019404No Hit
ACAGGTGAACTTCTAGATCCATGTGGTT1330.11948397297685782No Hit
GAGTGGGACCAACTTCCTGTTGATGTTCA1330.11948397297685782No Hit
ACTCCAGTATATGCTGATGGACCAGGAAG1330.11948397297685782No Hit
ACTTGGAGCCAGATTCCTCCTGAAGACAC1330.11948397297685782No Hit
ATCACCTATGAAGATAGACCATCTAAAG1320.11858559724018973No Hit
ACTGCTGTGAATGTGGATTCTTCCCACAC1320.11858559724018973No Hit
TACAGAACTGTACAACTCGTGTGGAAGAC1290.11589047003018542No Hit
TTGACGTTGCAGACTTGGCTAGTGCAAG1290.11589047003018542No Hit
AGTCCAGATATTTCACATTTTGAAAGGTC1280.11499209429351731No Hit
CTGTCTAGTATCTTAAAATTGACATGGAC1270.1140937185568492No Hit
ACAAAACTGACAGTAAATCTCACAAATG1270.1140937185568492No Hit
TACAGACACCAAGTTCCGTCAGTCCAAGT1270.1140937185568492No Hit
CCTACTGTTCGGACAAAAAAAGTAGGGA1260.11319534282018111No Hit
TATCCGCGCAAGATGTTGACGTTGCAGAC1260.11319534282018111No Hit
AAGAAGATGGTACCGTGCATCGCAC1250.112296967083513No Hit
GAGTGGGACCAACTTCCTGTTGATGTTC1250.112296967083513No Hit
TGGACCAACCCAAGTATTAAGAGTGTTAT1240.11139859134684489No Hit
GAGTGAAACCCAATGGAAAAAGCATGAC1230.1105002156101768No Hit
AGGCTTGCCTCCAGAAAAACCTAAAA1220.10960183987350869No Hit
GATTATGAAGTGACTCTCACAAGATGGA1210.10870346413684058No Hit
TGTAAAGAAATATATACTTGAGTGGTG1210.10870346413684058No Hit
TCATCAGTCAATGAGGAAGATTTTGTTAG1190.10690671266350438No Hit
CCAAAGTTTGCTCAAGGAGAAATTGAAG1180.10600833692683627No Hit
TCTAAAGCACCAAGTTTCTGGTATAAAA1170.10510996119016816No Hit
AAAGGACCTACTGTTCGGACAAAAA1170.10510996119016816No Hit
ATTATGAAGTGACTCTCACAAGATGGA1170.10510996119016816No Hit
GTGCTGTTCTGCTTTAATAAGCGAGACC1160.10421158545350007No Hit
AGTGGCTACAGACACCAAGTTCCGTCAGT1150.10331320971683196No Hit
GTGGTACACAGTGGCTACAGACACCAAGT1150.10331320971683196No Hit
AAAAACGAAGCTGTCTTAGAGTGGGACC1140.10241483398016385No Hit
ATCACCTATGAAGATAGACCATCTAAAGC1140.10241483398016385No Hit
AAGCGAGACCTAATTAAAAAACACATCT1140.10241483398016385No Hit
AGCACTGTCCAGTATTCTACCGTGGTAC1140.10241483398016385No Hit
TTCGGACAGCTTGAACAGAATGTTTATGG1140.10241483398016385No Hit
AAGGATCTGACAGTGTTCCGGAGCCGGG1130.10151645824349576No Hit
AGAAATCTTGTTGGCAAATCAGATGCAGC1120.10061808250682765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)