FastQCFastQC Report
Wed 28 Jun 2023
SRR1288394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases208 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA446941.1173499999999998No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA304120.7603000000000001No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA167470.418675No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA134180.33544999999999997No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT123220.30805No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA117990.294975No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA111740.27935No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA94970.237425No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT86870.217175No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA83330.208325No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA82850.207125No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG81670.20417500000000002No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG71790.179475No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT67540.16885No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA66240.1656No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA63900.15975No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAGAT63180.15795No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC60020.15005000000000002No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG54070.13517500000000002No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA53120.1328No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAG52670.131675No Hit
ATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAAAAAAGATCG43090.10772500000000002No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA42250.105625No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAC40110.10027499999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)