FastQCFastQC Report
Wed 28 Jun 2023
SRR1288395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases208 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA464501.16125No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA322560.8064No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA186620.46655No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA123640.3091No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA123120.3078No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA121300.30325No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT110460.27615No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT110430.276075No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA104940.26234999999999997No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA95560.2389No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA88500.22125No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT74690.186725No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA70340.17585No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG70130.175325No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA63690.159225No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG60970.152425No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAGAT59770.149425No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC56840.1421No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA56330.140825No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG45530.113825No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAG44810.112025No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA42480.1062No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA42320.10579999999999999No Hit
ATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAAAAAAGATCG41010.10252499999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)