FastQCFastQC Report
Wed 28 Jun 2023
SRR1288396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300685
Total Bases119.6 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA253381.10132417084477No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA164880.7166561263275938No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA94690.41157307497549644No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA81110.3525471761670981No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT76000.33033639981136054No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA63940.27791722899918936No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA61830.2687460473728477No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA54440.2366251790227693No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT52140.2266281563968992No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG46860.20367846967316258No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA46260.2010705507272834No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA44680.19420303083646828No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG41940.18229353431695344No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT40690.17686036984637185No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC37920.16482047737956304No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAGAT37710.16390770574850536No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA36640.1592569169616875No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA35880.15595355296357388No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG31050.13495980544924663No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAG29500.12822268150572547No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA29490.1281792161899608No Hit
ATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAAAAAAGATCG26880.11683476877538647No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA23880.10379517404599066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)