FastQCFastQC Report
Wed 28 Jun 2023
SRR1288400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20351396
Total Bases732.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAA2853191.401962794100218No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA2364031.1616058180971958No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAA2340741.1501618857006173No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA2184971.0736216817755402No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAA2029870.997410693595663No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAA2005630.9854999627543979No Hit
CACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAA1873210.9204331732329321No Hit
TCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAA1756840.8632528205927494No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA1724630.8474258964839563No Hit
AATATATTAGCATGGAATAATAGAATAAAAAAAAAA1665860.8185482705952948No Hit
CACGTTCTAGCATTCAAGGCCAAAAAAAAAAAAAAA1662880.8170839975793307No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAA1516970.745388670143316No Hit
TCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAA1283510.6306741807785569No Hit
ATATTAGCATGGAATAATAGAATAAAAAAAAAAAAA1086880.5340567300641195No Hit
AATATATTAGCATGGAATAATAGAAAAAAAAAAAAA1024210.5032627737183237No Hit
TACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAA1010070.49631484739425247No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAA930500.4572167924008751No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAA886800.435744063945294No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAA814040.39999221675014335No Hit
TATTAGCATGGAATAATAGAATAAAAAAAAAAAAAA806660.39636592988510466No Hit
TCACGTTCTAGCATTCAAGGCCAAAAAAAAAAAAAA739200.36321832664452114No Hit
TACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAA673360.330866737593824No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAA670960.32968745731251065No Hit
CTCGAATATATTAGCATGGAATAATAAAAAAAAAAA664990.3267539976127436No Hit
ACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAA641400.31516265518100084No Hit
CTCGAATATATTAGCATGGAATAATAGAAAAAAAAA620020.30465723334163414No Hit
TTCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAAA596040.292874257864178No Hit
CACGTTCTAGCATTCAAGGACAAAAAAAAAAAAAAA595700.2927071931576586No Hit
CACGTTCTAGCATTCAAGGGCAAAAAAAAAAAAAAA539410.26504815689302097No Hit
AATATATTAGCATGGAATAATAAAAAAAAAAAAAAA533520.26215400653596443No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAA530110.26047844580293167No Hit
ATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAA528400.25963820860249587No Hit
AATATATTAGCATGGAATAATAGAACAAAAAAAAAA520700.25585468436661546No Hit
TCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAAAA510490.2508378295031948No Hit
CTCGAATATATTAGCATGGAATAATAGAATAAAAAA499540.24545736321970246No Hit
ATGTACACGGAGTCGACCCGCAAAAAAAAAAAAAAA469150.2305247266575718No Hit
ACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAA467830.22987612250284944No Hit
CACGTTCTAGCATTCAAGGTCTAAAAAAAAAAAAAA464000.22799418772058686No Hit
ACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA463040.2275224756080615No Hit
CTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA458250.2251688287132735No Hit
CACGTTCTAGCATTCAAGGTAAAAAAAAAAAAAAAA452960.22256949842654528No Hit
ACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAA395780.194473145724254No Hit
ATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAA394020.1936083401846242No Hit
ACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAA389420.19134805297877353No Hit
CCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAA383700.18853743497497666No Hit
CACGTTCTAGCATTCAAGGTCGAAAAAAAAAAAAAA382970.18817873722274384No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA363080.1784054518913592No Hit
TGTGGCGTCGCTGAACCATAGAAAAAAAAAAAAAAA353390.17364410775555641No Hit
CACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAA351440.1726859425269893No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAA349710.1718358779908759No Hit
GAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAA348010.17100055445827894No Hit
TGCTCGAATATATTAGCATGGAATAATAAAAAAAAA337380.1657773255456284No Hit
AGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA336690.16543828246475084No Hit
GAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAA331570.16292248453128227No Hit
TCACGTTCTAGCATTCAAGGTCTAAAAAAAAAAAAA328080.16120761445553908No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAA325990.16018065787722868No Hit
AGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAA322470.15845104679796906No Hit
ACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA320980.157718910289987No Hit
CGAATATATTAGCATGGAATAATAGAATAAAAAAAA318170.15633816962728256No Hit
ATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA314820.15469209090128264No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAA310120.15238266701704395No Hit
AGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAA302550.14866302046306798No Hit
TCACGTTCTAGCATTCAAGGACAAAAAAAAAAAAAA296230.14555758238894276No Hit
ATATTAGCATGGAATAATAGAACAAAAAAAAAAAAA294990.14494828757693085No Hit
CACGTTCTAGCATTCAAGGTCCTAAAAAAAAAAAAA286310.14068322389284743No Hit
TGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAA281740.1384376776905132No Hit
ATATATTAGCATGGAATAATAGAATAAAAAAAAAAA281640.13838854101212517No Hit
TGCTCGAATATATTAGCATGGAATAAAAAAAAAAAA278640.13691444066048344No Hit
CCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAA276160.13569585103645962No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT271250.13328324012760598No Hit
TCACGTTCTAGCATTCAAGGTAAAAAAAAAAAAAAA265520.1304677084559703No Hit
TCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAA262730.12909679512894348No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAA260140.1278241551586928No Hit
GCATGGAATAATAGAATAAAAAAAAAAAAAAAAAAA256570.12606997574023915No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAA256350.12596187504778542No Hit
GCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAA255460.1255245586101317No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAA254720.12516094719006007No Hit
GAATATATTAGCATGGAATAATAGAATAAAAAAAAA254670.12513637885086606No Hit
TTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAA249280.12248791188574976No Hit
TACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAA248850.12227662416868111No Hit
GCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA248850.12227662416868111No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAA248830.12226679683300348No Hit
CACGTTCTAGCATTCAAGGTCCGAAAAAAAAAAAAA246180.12096467485571998No Hit
TATATTAGCATGGAATAATAGAATAAAAAAAAAAAA244170.11997702762012001No Hit
ACGTTCTAGCATTCAAGGCCAAAAAAAAAAAAAAAA238770.1173236469871649No Hit
TCACGTTCTAGCATTCAAGGTCGAAAAAAAAAAAAA230250.1131372019885024No Hit
AATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAA229540.11278833157194719No Hit
TGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAA228410.11223308710616214No Hit
TCACGTTCTAGCATTCAAGGGCAAAAAAAAAAAAAA227560.11181542533986366No Hit
TTTCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAA222760.10945686477723691No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAA218850.10753562065226385No Hit
ATTCATAATAACTTTTCGAATCGCATAAAAAAAAAA214020.10516231908612068No Hit
CGAATATATTAGCATGGAATAATAGAAAAAAAAAAA213890.10509844140421619No Hit
TCGAATATATTAGCATGGAATAATAGAATAAAAAAA212340.1043368228892013No Hit
TTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA212320.10432699555352369No Hit
TCACGTTCTAGCATTCAAGGTCCTAAAAAAAAAAAA210150.10326072963250287No Hit
CGAATATATTAGCATGGAATAATAAAAAAAAAAAAA203900.10018968723324927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)