FastQCFastQC Report
Tue 27 Jun 2023
SRR1288401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases208 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA539751.349375No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA533501.33375No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA217760.5444No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA163060.40765No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA162130.40532500000000005No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA153610.384025No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA133940.33485No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA124350.310875No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA115000.2875No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA114380.28595No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA114020.28505No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA96950.242375No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT93220.23305No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA84700.21175No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA84330.21082499999999998No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAT79290.198225No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA77590.193975No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA76830.192075No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA73900.18475No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT73430.183575No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA72630.18157500000000001No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA71730.17932499999999998No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAA69520.1738No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA66780.16695No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAAA66360.16590000000000002No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG65830.164575No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAA64300.16075No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA63760.1594No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAA62900.15725No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA61880.1547No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA60290.150725No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA59590.148975No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA57470.143675No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAC57400.14350000000000002No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATCG52460.13115No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA52100.13025No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAA51540.12885No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATC48460.12115No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA47480.11869999999999999No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAGA46970.117425No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAAAA46800.117No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA46700.11674999999999999No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA46320.1158No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAAAAAAAAAAAAAAAAAAA45780.11445No Hit
AGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAAAAAAAAAAAAAAAAAA45430.11357500000000001No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA44090.11022499999999999No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAAAAAAAAAAAAAAAAAAAAA43820.10955No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAAA42550.106375No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG42380.10594999999999999No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA42310.10577500000000001No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAA41580.10395No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATCG41190.10297500000000001No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAA41070.10267500000000002No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA40610.101525No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT40480.10120000000000001No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC40450.10112499999999999No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCTAAAAAAAAAAAAAAAAAAAA40270.100675No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAT40150.100375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)