FastQCFastQC Report
Wed 28 Jun 2023
SRR1288402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases208 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA530571.326425No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA524461.31115No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA196870.492175No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA148010.370025No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA146480.36619999999999997No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA144900.36224999999999996No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA134560.3364No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA111160.2779No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA110690.276725No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA104280.2607No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA102770.256925No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT96690.24172500000000002No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT90710.22677499999999998No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA83960.20990000000000003No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA83090.20772500000000002No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAT81340.20335No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA78060.19515No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA76690.19172499999999998No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA76170.19042499999999998No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG75020.18755No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA73090.182725No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA70750.176875No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA69530.173825No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA68760.1719No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA63340.15835000000000002No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA62600.1565No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAAA62100.15525No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA60160.15039999999999998No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAA59410.148525No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAA59300.14825000000000002No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAA58350.145875No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA58220.14554999999999998No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA58110.14527500000000002No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAGA56670.141675No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA52710.131775No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAA50640.1266No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATCG50520.1263No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG50150.12537500000000001No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA47540.11885000000000001No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATC46520.1163No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAAAAAAAAAAAAAAAAAAA46020.11505No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC46010.115025No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATCG45910.11477499999999999No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAC45780.11445No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA45660.11414999999999999No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA44860.11215000000000001No Hit
AGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAAAAAAAAAAAAAAAAAA44800.11199999999999999No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA44180.11045No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAAAA42810.10702500000000001No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT41760.1044No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAGAT41240.1031No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG40850.10212500000000001No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA40570.101425No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA40470.101175No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAT40060.10015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)