FastQCFastQC Report
Wed 28 Jun 2023
SRR1288403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases208 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA534451.336125No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA507801.2695No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA181400.4535No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA140570.351425No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA140080.3502No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA139950.349875No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA134310.335775No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA105560.26389999999999997No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT105230.263075No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA99910.249775No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA99820.24955No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA96990.242475No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT91100.22775No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAT85820.21455No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA84480.21120000000000003No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG81650.204125No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA79760.19940000000000002No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA76420.19105No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA76170.19042499999999998No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA75630.189075No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA71330.17832499999999998No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA70350.175875No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA70130.175325No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA67600.169No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAGA61640.15410000000000001No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA60530.15132500000000002No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA60040.15009999999999998No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAA58290.145725No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA57640.1441No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAA57050.142625No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAA56240.1406No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA56220.14055No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAAA56000.13999999999999999No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA55160.1379No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG53170.132925No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA52380.13094999999999998No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAA50880.12719999999999998No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAGAT47550.118875No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA47460.11865No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATCG47140.11785000000000001No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC47040.1176No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATCG46520.1163No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAAAAAAAAAAAAAAAAAAA45910.11477499999999999No Hit
AGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAAAAAAAAAAAAAAAAAA45740.11435No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT45320.11329999999999998No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA45020.11255No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATC44710.111775No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA44220.11055No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA44140.11035No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATGG43800.1095No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG43220.10805000000000001No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAT41470.10367499999999999No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAA41290.103225No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAAAA41010.10252499999999999No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAGATCG40750.101875No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA40290.100725No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA40180.10045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)