FastQCFastQC Report
Wed 28 Jun 2023
SRR1288404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1288404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637998
Total Bases85.1 Mbp
Sequences flagged as poor quality0
Sequence length52
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA214211.307754954523754No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA205091.2520772308635297No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA67280.41074531226533856No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA54970.33559259535115427No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA53400.32600772406315515No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA52750.3220394652496523No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA52020.31758280535141065No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT49600.3028086725380617No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA42310.2583031236912377No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA37350.22802225643743157No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA36680.22393189735274402No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA36160.22075729030194174No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA36120.22051308975957234No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA35270.21532382823422253No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG35240.21514067782744548No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT32480.19829084040395656No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA32120.19609303552263188No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAT31890.19468888240400783No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA30970.1890722699295115No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA30170.1841882590821234No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAGA28410.17344343521786962No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA27620.16862047450607387No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA27400.16727737152304215No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA26290.16050080647229117No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAAA26120.1594629541672212No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG25070.15305268993002433No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA24870.1518316872181773No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA23250.1419415652522164No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAGAT23120.14114791348951586No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATCG22360.1365081031844972No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA22360.1365081031844972No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAA22280.13601970209975836No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA22240.13577550155738896No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAA22200.13553130101501956No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC21750.13278404491336374No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAGATCG21550.13156304220151674No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA20940.12783898393038332No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAAAAA20530.12533592837109692No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA20460.12490857742195045No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAAA20140.12295497308299523No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAA19750.12057401779489352No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG19740.12051296765930117No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATCG19500.11904776440508474No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA19410.11849831318475358No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAAAAAAAAAAAAAAAAAAA19210.11727731047290657No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAGATC19110.11666680911698304No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAGA19060.11636155843902128No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA18390.11227119935433376No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAGATGG18000.10989024406623207No Hit
AGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAAAAAAAAAAAAAAAAAA17980.10976814379504737No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA17940.10952394325267796No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT17200.10500623321884398No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAGATCGG16930.1033578795578505No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA16880.10305262887988875No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAGATCGGA16740.10219792698159583No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAAAA16710.10201477657481876No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA16600.10134322508330292No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATC16430.10030537277823295No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCTAAAAAAAAAAAAAAAAAAAA16400.10012222237145588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)