FastQCFastQC Report
Fri 29 Sept 2023
SRR12925971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12925971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397361
Total Bases341.6 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN820571087.31930166352022No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1418751.5097323599678676No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1301361.3848143111667202No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG415190.44181552672074637No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGA396350.4217673451089088No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC279210.2971153284416763No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC239830.2552099467073788No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG230960.2457711265960731No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC182410.19410768618977178No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG158490.1686537316167805No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC146830.1562459928909829No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG136190.1449236652715587No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC113890.12119359892633687No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG94330.10037924476882393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)