FastQCFastQC Report
Fri 29 Sept 2023
SRR12925975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12925975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8629649
Total Bases318.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN776374089.96588389632069No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1240771.4377989185886935No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC276740.3206851170887715No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC186170.21573299215298328No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG158640.18383134702234125No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG148840.17247514933689656No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG143640.16644941178951775No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC140550.16286873313155611No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC122270.14168594806115523No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC97780.11330704180436539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86720.10049076155936353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)