FastQCFastQC Report
Fri 29 Sept 2023
SRR12925979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12925979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9066578
Total Bases336.3 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN807079289.01695876878796No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC891440.9832154976221459No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC890550.9822338703753499No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC251750.277668156607708No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG210920.23263462797099413No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG166430.1835642951508276No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC160340.17684731769803336No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG146030.16106407511191104No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG124880.13773664110097547No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC107710.11879895590155404No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC98560.10870694544292234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)