FastQCFastQC Report
Fri 29 Sept 2023
SRR12925991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12925991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7061459
Total Bases266 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN632604589.58552333165143No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC477270.6758801545119784No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC290240.4110198756375984No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG161440.22862130899577554No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG122750.17383093210624037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90970.12882606838048624No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC89580.1268576366442119No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC70850.10033337303240024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)