FastQCFastQC Report
Wed 6 Sept 2023
SRR12993020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7618246
Total Bases220.8 Mbp
Sequences flagged as poor quality0
Sequence length25-51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAAC1778232.334172459119855No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1270941.668284274359216No Hit
TTAATCAGTGAAATTGACCTTTCAGTG860071.1289606557729954No Hit
ATGTACACGGAGTCGACCCAACGCGA794081.0423396671622314No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAA727740.9552592552143891No Hit
TTTTTAGGTAGAGGTGAAAAGCCTA322660.4235358112615424No Hit
TGATCAACGGACCAAGTTACCCTAGGGATA322240.4229845032570489No Hit
TCGGTTTCTATCTATTTACGATTTCTC300040.3938439373052537No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT296650.38939409412612824No Hit
TGATCAACGGACCAAGTTACCCTAGGGA227660.29883519119755386No Hit
TAATCAGTGAAATTGACCTTTCAGTG223780.2937421553465194No Hit
CGGTTTCTATCTATTTACGATTTCTC216870.2846718260344967No Hit
TTTTAGGTAGAGGTGAAAAGCCTAA198000.2599023449754707No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTG173830.22817588195498018No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT166220.21818670596880171No Hit
AGGAGTGGAGCCTGCGGCTTAATTTG158120.2075543373107143No Hit
GATCAACGGACCAAGTTACCCTAGGGATA148450.1948611268263062No Hit
ACCTCTAGCATTACAAGTATTAGAGGC141620.18589580856275842No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATA124130.16293776808992516No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT120950.15876357891304638No Hit
TTGATCAACGGACCAAGTTACCCTAGGGA114080.14974575512526112No Hit
AGAGCCACCTTACAAATAAGCGCTCTC109460.14368136707583345No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTT97750.12831037485531446No Hit
ATTTTTAGGTAGAGGTGAAAAGCCTAA91570.12019827136062554No Hit
GATTTTTAGGTAGAGGTGAAAAGCCTAA89970.11809805039112678No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTA86510.11355632254458572No Hit
CCTCTAGCATTACAAGTATTAGAGGC84600.11104918376224658No Hit
GACAAAGACAAAAGTGGCTTCATTGAGG84190.11051100213881253No Hit
ACGATTAAAGTCCTACGTGATCTGAGT81040.10637619210511184No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGC80440.10558860924154982No Hit
ATGGAGTCTGCGGGGATCCATGAGACCA80130.10518169142870945No Hit
TGATCAACGGACCAAGTTACCCTAGGG78830.10347526189099171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)