FastQCFastQC Report
Wed 6 Sept 2023
SRR12993021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9321836
Total Bases267 Mbp
Sequences flagged as poor quality0
Sequence length25-51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2299412.466692183814433No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC2022462.169594058509504No Hit
CGGTTTCTATCTATTTACGATTTCTC1772901.9018785569709658No Hit
TCGGTTTCTATCTATTTACGATTTCTC1604451.7211738116825914No Hit
ATGTACACGGAGTCGACCCAACGCGA842070.9033306314335502No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTT700770.7517510499004703No Hit
TTAATCAGTGAAATTGACCTTTCAGTG537560.5766675148543698No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT509980.547081068579194No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAA455210.4883265485468743No Hit
CTCTAGCATTACAAGTATTAGAGGC435480.4671611901346473No Hit
ACCTCTAGCATTACAAGTATTAGAGGC366400.39305561694069713No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT337190.36172058809015734No Hit
TTTTTAGGTAGAGGTGAAAAGCCTA289360.31041095337871205No Hit
TCGGTTTCTATCTATTTACGATTTCT277910.2981279653493153No Hit
ACGATTAAAGTCCTACGTGATCTGAG271120.2908439925353761No Hit
AGAGCCACCTTACAAATAAGCGCTCTC269310.2889023149516898No Hit
ACGATTAAAGTCCTACGTGATCTGAGT256230.2748707443469291No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTG244120.26187974128701685No Hit
CCTCTAGCATTACAAGTATTAGAGGC244010.26176173878193093No Hit
AGGAGTGGAGCCTGCGGCTTAATTTG227830.24440464303384013No Hit
GGAATGCCTAAAGGAAAGATCCAAAA215190.23084508244942303No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT205360.22029994949492784No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGA202300.21701733435344708No Hit
TAAGGAGAGGGTATCAAGCACATTAA195140.20933644402240073No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAAT191580.2055174538578023No Hit
TGATCAACGGACCAAGTTACCCTAGGGA177600.1905204082114296No Hit
TTAAGGAGAGGGTATCAAGCACATTAA167080.17923507772503183No Hit
TAATCAGTGAAATTGACCTTTCAGTG159500.17110363237456655No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATAC154750.1660080696549478No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAA151760.16280054701670357No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTA143390.1538216291297122No Hit
TTTTAGGTAGAGGTGAAAAGCCTAA143050.15345689411399216No Hit
CGATTAAAGTCCTACGTGATCTGAG138040.14808241638235214No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTG132720.14237538613637915No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGC131420.14098081107627294No Hit
TTGATCAACGGACCAAGTTACCCTAGGGA116950.12545811790724487No Hit
AACGATTAAAGTCCTACGTGATCTGAGT107670.11550299747817919No Hit
CGGTTTCTATCTATTTACGATTTCT105200.11285330486397745No Hit
ATAACCTAGACTTACAAGTCAAAGTAAA102950.1104396172599475No Hit
AAGGAGAGGGTATCAAGCACATTAA99870.10713554711754208No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTG99610.10685663210552085No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC99370.10659917209442432No Hit
CGACTGCATAATTTGTGGTAGTGGGG97530.10462531200935093No Hit
CGGAATGCCTAAAGGAAAGATCCAAAA96010.10299473193907294No Hit
AATGCCTAAAGGAAAGATCCAAAAAG95610.10256563192057874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)