FastQCFastQC Report
Wed 6 Sept 2023
SRR12993022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9946729
Total Bases285.1 Mbp
Sequences flagged as poor quality0
Sequence length25-51
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAAC2453202.466338431458221No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1778541.788065202138311No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAA1208891.2153643675222277No Hit
CCTCTAGCATTACAAGTATTAGAGGC1151061.1572246514406896No Hit
TTAATCAGTGAAATTGACCTTTCAGTG1063711.069406837162247No Hit
CGGTTTCTATCTATTTACGATTTCTC781150.7853335503561021No Hit
CTCTAGCATTACAAGTATTAGAGGC712020.7158333156558302No Hit
TTTTTAGGTAGAGGTGAAAAGCCTA588030.5911792710950504No Hit
AGGAGTGGAGCCTGCGGCTTAATTTG573610.5766820429107901No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT477970.4805298304598426No Hit
ATGTACACGGAGTCGACCCAACGCGA477430.4799869384196553No Hit
TCGGTTTCTATCTATTTACGATTTCTC464550.4670379579055587No Hit
ACCTCTAGCATTACAAGTATTAGAGGC370960.372946724496063No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTG357430.35934426282248166No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTG337030.3388350079709621No Hit
TGATCAACGGACCAAGTTACCCTAGGGA326720.32846979142590493No Hit
AGAGCCACCTTACAAATAAGCGCTCTC307690.30933787378745314No Hit
TAATCAGTGAAATTGACCTTTCAGTG296340.29792708738722046No Hit
TTTTAGGTAGAGGTGAAAAGCCTAA278920.28041379231303076No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT277180.27866447351687174No Hit
TGATCAACGGACCAAGTTACCCTAGGGATA237810.23908362236469896No Hit
GATTTTTAGGTAGAGGTGAAAAGCCTAA207470.20858113255121358No Hit
ATTTTTAGGTAGAGGTGAAAAGCCTAA196600.1976529168533696No Hit
CCTCTAGCATTACAAGTATTAGAGG193030.19406379725435366No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT187250.1882528417130898No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATA176040.17698280510105383No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTG170730.17164436670587888No Hit
TTGATCAACGGACCAAGTTACCCTAGGGA164750.16563234003861974No Hit
CAGGAGTGGAGCCTGCGGCTTAATTTGA155360.15619205067314088No Hit
GATCAACGGACCAAGTTACCCTAGGGATA153510.15433214275768448No Hit
CGGTTTCTATCTATTTACGATTTCT151890.15270346663712261No Hit
TCGGTTTCTATCTATTTACGATTTCT147070.14785765250063615No Hit
ACGATTAAAGTCCTACGTGATCTGAG142720.14348435551023858No Hit
AGATTTTTAGGTAGAGGTGAAAAGCCTAA140980.14173503671407958No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTT137150.13788452465126977No Hit
ACGATTAAAGTCCTACGTGATCTGAGT135190.13591402761651594No Hit
CGATTAAAGTCCTACGTGATCTGAG135070.13579338494091878No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTA122820.12347777847370729No Hit
GATTTTTAGGTAGAGGTGAAAAGCCTA110460.1110515828871984No Hit
AATAGCTTAAGACACCTTGCCTAGC104120.10467762819314771No Hit
TGATCAACGGACCAAGTTACCCTAGGG102060.10260659559539623No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAA100670.10120915126972897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)