FastQCFastQC Report
Wed 6 Sept 2023
SRR12993026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7947156
Total Bases226.6 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGG2419343.044284018081437No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1847252.324416432746507No Hit
AATGGATTTTTGGAGCAGGGAGATGGA1306151.643543929425822No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1239431.5595893675674668No Hit
GACTGCATAATTTGTGGTAGTGGGGG1118901.4079250489105788No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA872991.0984935994713076No Hit
GACTGCATAATTTGTGGTAGTGGGGGA563540.7091090196291604No Hit
ATGGATTTTTGGAGCAGGGAGATGGA540140.6796645240133703No Hit
TTAAATGGATTTTTGGAGCAGGGAG511420.6435258097362125No Hit
GTCTAGGGGTATGATTCTCGCTT477590.6009571222711622No Hit
TAAATGGATTTTTGGAGCAGGGAGA438900.5522730395628322No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA399450.5026326399028784No Hit
TGGATTTTTGGAGCAGGGAGATGGA352780.4439072292024971No Hit
AATGGATTTTTGGAAATAGGAGATGGA338280.4256617084149349No Hit
TGACTGCATAATTTGTGGTAGTGGGGG322580.4059062134932295No Hit
TTAAATGGATTTTTGGAGCAGGGAGA319510.40204319633338015No Hit
TAAATGGATTTTTGGAGCAGGGAG312070.392681356701693No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG279740.35200013690432147No Hit
AAATGGATTTTTGGAAATAGGAGATGGA267950.3371646410363657No Hit
ACTGCATAATTTGTGGTAGTGGGGG256360.32258080752410045No Hit
GCTGGTCCGATGGTAGTGGGTTATC252210.31735881364352225No Hit
AATGGATTTTTGGAGCAGGGAGATGG238630.3002709396921364No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC226280.2847307892282472No Hit
GTGAGACGAATTTTTGAGCGGGTAA203270.25577703520605355No Hit
AAATGGATTTTTGGAGCAGGGAGATGG177560.22342583938203806No Hit
ATGGATTTTTGGAAATAGGAGATGGA173980.21892108321517784No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA166700.20976057346804317No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA162700.20472732635423285No Hit
ATGTACACGGAGTCGACCCAACGCGA148690.1870983783381124No Hit
CTCGACTGCATAATTTGTGGTAGTTGGGG141390.17791270235540865No Hit
ACTGCATAATTTGTGGTAGTGGGGGA141200.17767362311750268No Hit
TCGACTGCATAATTTGTGGTAGTGGGG140950.17735904517288953No Hit
TTAAATGGATTTTTGGAGCAGGGA131010.16485142609507097No Hit
GTCTAGGGGTATGATTCTCGCT125580.15801879313807354No Hit
TGACTGCATAATTTGTGGTAGTGGGGGA119990.15098483029652368No Hit
GGATTTTTGGAGCAGGGAGATGGA116280.14631649359846466No Hit
ATTAAATGGATTTTTGGAGCAGGGAG113150.14237797773190813No Hit
TGGATTTTTGGAAATAGGAGATGGA109400.137659308562711No Hit
AAATGGATTTTTGGAGCAGGGAGA108620.13667782537551798No Hit
ATTAAATGGATTTTTGGAGCAGGGAGA103540.13028560154097893No Hit
ATGGATTTTTGGAGCAGGGAGATGG102360.1288007936424049No Hit
AGGTGGATTTAGCAGTAAACTGAGAGT98520.12396887641314704No Hit
CTGCATAATTTGTGGTAGTGGGGG98200.12356621664404221No Hit
TAAATGGATTTTTGGAGCAGGGAGATGG95380.12001777742880598No Hit
CTGCATAATTTGTGGTAGTGGGGGA94370.11874688253256888No Hit
GACTGCATAATTTGTGGTAGTGGGG91960.1157143511464982No Hit
GTGAGACGAATTTTTGAGCGGGTA86730.10913338054519126No Hit
CGACTGCATAATTTGTGGTAGTGGGG84440.10625184657253488No Hit
GGCTGGTCCGATGGTAGTGGGTTATC82250.10349614377772375No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGC79540.10008611885811729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)