FastQCFastQC Report
Tue 5 Sept 2023
SRR12993027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4792155
Total Bases141.2 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGG1095252.2855062075412835No Hit
AATGGATTTTTGGAGCAGGGAGATGGA693521.4471985985428268No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG665071.3878307358589195No Hit
CGACTGCATAATTTGTGGTAGTGGGGG573311.1963511196945842No Hit
GACTGCATAATTTGTGGTAGTGGGGG476390.9941039052367879No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA433330.9042487148266282No Hit
ATGGATTTTTGGAGCAGGGAGATGGA279330.5828901611070594No Hit
GACTGCATAATTTGTGGTAGTGGGGGA253620.5292399765867339No Hit
TTAAATGGATTTTTGGAGCAGGGAG197970.41311268103807164No Hit
AATGGATTTTTGGAAATAGGAGATGGA194150.405141319510742No Hit
TTAAATGGATTTTTGGAGCAGGGAGA180870.37742936111206754No Hit
TAAATGGATTTTTGGAGCAGGGAGA169340.3533692044602063No Hit
TGACTGCATAATTTGTGGTAGTGGGGG168060.3506981723253943No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG165220.3447718197762802No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA163070.34028532048733817No Hit
AAATGGATTTTTGGAAATAGGAGATGGA141860.2960254833159612No Hit
TGGATTTTTGGAGCAGGGAGATGGA138060.28809585666573806No Hit
AATGGATTTTTGGAGCAGGGAGATGG119940.2502840580073057No Hit
AAATGGATTTTTGGAGCAGGGAGATGG97000.20241415396622187No Hit
ATGGATTTTTGGAAATAGGAGATGGA96160.2006612891277515No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG87440.18246488270934474No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC85610.1786461414541057No Hit
GTGAGACGAATTTTTGAGCGGGTAA80620.16823328961604955No Hit
GCTGGTCCGATGGTAGTGGGTTATC76850.1603662652814861No Hit
TCGACTGCATAATTTGTGGTAGTGGGG76140.15888467714420756No Hit
ACTGCATAATTTGTGGTAGTGGGGG72530.1513515318264956No Hit
AGGTGGATTTAGCAGTAAACTGAGAGT69820.14569645597857334No Hit
CTCGACTGCATAATTTGTGGTAGTTGGGG66680.13914408027286262No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA65530.13674432483924248No Hit
ATTAAATGGATTTTTGGAGCAGGGAG60980.12724964029752794No Hit
ATTAAATGGATTTTTGGAGCAGGGAGA59100.12332656184952283No Hit
ACTGCATAATTTGTGGTAGTGGGGGA56370.11762975112449409No Hit
TGACTGCATAATTTGTGGTAGTGGGGGA55930.11671158382815247No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA54750.11424922607887265No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG53710.11207901246933791No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG52610.10978359422848384No Hit
TAAATGGATTTTTGGAGCAGGGAGATGG49650.10360683241673109No Hit
TGGATTTTTGGAAATAGGAGATGGA48520.10124881186021739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)