FastQCFastQC Report
Wed 6 Sept 2023
SRR12993028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7268938
Total Bases215.7 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAAC4258415.858366105199962No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG2455413.3779487457452517No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1814272.4959216875972805No Hit
AATGGATTTTTGGAGCAGGGAGATGGA830681.1427804171668543No Hit
CGACTGCATAATTTGTGGTAGTGGGGG787641.0835695668335594No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA727291.00054505898936No Hit
GACTGCATAATTTGTGGTAGTGGGGG501890.6904584961379503No Hit
GACTGCATAATTTGTGGTAGTGGGGGA368230.5065801909439867No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA363460.5000180218898552No Hit
ATGGATTTTTGGAGCAGGGAGATGGA295780.406909510027462No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA292980.4030575030355191No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA257590.35437088609092554No Hit
AATGGATTTTTGGAAATAGGAGATGGA249900.3437916240309107No Hit
AAATGGATTTTTGGAAATAGGAGATGGA224700.3091235611034239No Hit
TTAAATGGATTTTTGGAGCAGGGAGA221220.3043360666991519No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG202120.2780598761469695No Hit
ATGTACACGGAGTCGACCCAACGCGA191160.2629820202070784No Hit
TGACTGCATAATTTGTGGTAGTGGGGG182150.25058681199371907No Hit
CTCGACTGCATAATTTGTGGTAGTTGGGG154550.21261702878742397No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG143390.19726402949096553No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG135060.1858043086899352No Hit
TGTACACGGAGTCGAGCTCAACCCGCAAC134250.1846899780958374No Hit
TTAAATGGATTTTTGGAGCAGGGAG126690.17428955921759134No Hit
AAATGGATTTTTGGAGCAGGGAGATGG123940.1705063380647902No Hit
TAAATGGATTTTTGGAGCAGGGAGA119070.1638065973323751No Hit
TAAATGGATTTTTGGAAATAGGAGATGGA117370.16146787880155256No Hit
AGGTGGATTTAGCAGTAAACTGAGAGT112840.15523588177530198No Hit
ATGGATTTTTGGAAATAGGAGATGGA110640.15220930485306108No Hit
AATGGATTTTTGGAGCAGGGAGATGG107030.1472429672670203No Hit
TAAATGGATTTTTGGAGCAGGGAGATGG91230.1255066420981992No Hit
TGACTGCATAATTTGTGGTAGTGGGGGA88280.12144827758883071No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGC87290.12008631797382231No Hit
TACACGGAGTCGAGCTCAACCCGCAAC85950.11824285748482104No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG82380.11333154857009374No Hit
TCGACTGCATAATTTGTGGTAGTGGGG80960.1113780307384655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)