FastQCFastQC Report
Wed 6 Sept 2023
SRR12993029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12993029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8279734
Total Bases244.5 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGG2359822.8501157162778417No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG2352052.8407313568286128No Hit
AATGGATTTTTGGAGCAGGGAGATGGA1316851.5904496448798962No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA1144211.381940530939762No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1062971.2838214367756258No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC988281.19361322477268No Hit
GACTGCATAATTTGTGGTAGTGGGGG729950.881610447871876No Hit
GACTGCATAATTTGTGGTAGTGGGGGA496340.5994637025778847No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA450430.5440150613534203No Hit
ATGGATTTTTGGAGCAGGGAGATGGA418070.504931680172334No Hit
AATGGATTTTTGGAAATAGGAGATGGA403630.48749150636964905No Hit
TTAAATGGATTTTTGGAGCAGGGAGA381060.4602321765409372No Hit
AAATGGATTTTTGGAAATAGGAGATGGA374470.4522729836489916No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA326100.3938532324830725No Hit
TGACTGCATAATTTGTGGTAGTGGGGG260800.31498596452494726No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA252870.3054083621526972No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG246660.29790812120292753No Hit
TTAAATGGATTTTTGGAGCAGGGAG215690.26050353791559006No Hit
AAATGGATTTTTGGAGCAGGGAGATGG204700.2472301646405549No Hit
TAAATGGATTTTTGGAGCAGGGAGA192620.23264032395243617No Hit
AATGGATTTTTGGAGCAGGGAGATGG180830.21840073606229377No Hit
ATGGATTTTTGGAAATAGGAGATGGA162340.1960691007706286No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG155990.18839977226321522No Hit
AGGTGGATTTAGCAGTAAACTGAGAGT154810.1869746057059321No Hit
TAAATGGATTTTTGGAAATAGGAGATGGA152270.18390687430296673No Hit
ATTAAATGGATTTTTGGAGCAGGGAGA140680.16990884006660117No Hit
TAAATGGATTTTTGGAGCAGGGAGATGG139050.16794017778832027No Hit
CTCGACTGCATAATTTGTGGTAGTTGGGG138900.16775901254798767No Hit
ATTAAATGGATTTTTGGAGCAGGGAG137480.16604398160617237No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG133500.16123706389601405No Hit
TGACTGCATAATTTGTGGTAGTGGGGGA118050.14257704414175626No Hit
TCGACTGCATAATTTGTGGTAGTGGGG114500.13828946678721804No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGC110860.13389319028848026No Hit
ATGTACACGGAGTCGACCCAACGCGA110680.13367579200008117No Hit
ACTGCATAATTTGTGGTAGTGGGGGA98010.11837336803332088No Hit
TGGATTTTTGGAGCAGGGAGATGGA91440.11043833050675299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)