FastQCFastQC Report
Tue 27 Jun 2023
SRR1301050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8556647
Total Bases436.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA5805136.784351393717657No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAA3081723.6015509346125887No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGTAAAAAAAAAAAAAAAAA1609061.880479585052416No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTAAAAAAAAAAAAAAAA1480121.7297897178649535No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGAAAAAAAAAAAAAAAAAA1238021.4468517866870048No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAAA882301.0311281977625115No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCGAAAAAAAAAAAAAAAA870361.017174133746548No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA859851.004891285102681No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGCCAAAAAAAAAAAAAAAAA849050.992269518656081No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA668890.7817197554135399No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGTAAAAAAAAAAAAAAAAAA381010.44527955868694824No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTAAAAAAAAAAAAAAAAA376590.4401139839004694No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAA359270.419872410302774No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGATAAAAAAAAAAAAAAAAA306660.3583880461587348No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATAAAAAAAAAAAAAAA276990.3237132488929367No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAGAAAAAAAAAAAAAAA264220.30878917875191064No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAA259380.30313275749250845No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAA255570.29868007877384684No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAAAAAAAAAAAAAAAAAAA236790.2767322293417036No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA233450.2728288311998847No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAAAAAAAAAAAAAAAAAAAA230490.26936953224785365No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA216940.2535338900856843No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGCCAAAAAAAAAAAAAAAAAA189960.2220028476107522No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGTAAAAAAAAAAAAAAAAA181050.21158989029230724No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGAAAAAAAAAAAAAAAAAAA180370.2107951864790028No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCGAAAAAAAAAAAAAAAAA171540.20047572372682898No Hit
TATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAAAAAAAAAAAAAAAAAAA168640.1970865456995012No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTAAAAAAAAAAAAAAAA166920.19507641252467234No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA166870.1950179784207529No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA154530.18059644157343407No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAATAAAAAAAAAAAAAA140060.16368561189914693No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAA139320.16282078716113918No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGAAAAAAAAAAAAAAAAAA136740.15980558739889586No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAAAAAAAAAAAAAAAAAAA135720.15861353167893918No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGTAAAAAAAAAAAAAAAAAA133540.15606580474805143No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCAGAAAAAAAAAAAAAAAAAA129980.151905296548987No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAAAAAAAAAAAAA125930.14717213413151203No Hit
ATCAGGTAACATCAAATCGTACCCCAAACCGACAAAAAAAAAAAAAAAAA117060.13680592409620265No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAAAAAAAAAAAAAAAAAA115660.13516976918645818No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAATAAAAAAAAAAAAA110750.12943154018156877No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGAAAAAAAAAAAA110060.12862514954748047No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCGAAAAAAAAAAAAAAAA98330.11491650876797885No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGCCAAAAAAAAAAAAAAAAA97650.11412180495467442No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGAAAAAAAAAAAAAA96470.11274276010217554No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA95080.111118292013215No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCAAAAAAAAAAAAAAAAAAAA88370.10327643526722557No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAA87180.1018857035939428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)