FastQCFastQC Report
Tue 27 Jun 2023
SRR1301052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7184193
Total Bases718.4 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAA1310571.824241080383002No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAA779351.0848121702743787No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAAA569490.792698637132939No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCAAAAAAAAAAAAAAAA508920.7083885413434745No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAA458870.6387217047203493No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAA424910.591451259730912No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA255900.35619867116598897No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGAAAAAAAAAAAAAAAAAAA208260.2898864214811601No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA201850.2809640553921644No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA192830.26840871340733746No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAAA185170.2577464163337483No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAAAAAAAAAAAAAAAA182760.2543918293954519No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAAAAAAAAAAAAAAAAAA180690.251510503684965No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA179400.2497148949088645No Hit
AGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAAA175200.2438687268006302No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAAAAAAAAAAAAAAAAAA163320.22733242272305323No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA132200.18401510093061252No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAAAAAAAAAAAAAAAAAA130300.18137040583403036No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAAAAAAAAAAA116440.1620780510768572No Hit
AGCTGCATCCGGGGTTCGAATCCCCGCCTCACCGCCAAAAAAAAAAAAAA114850.15986485886445423No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA111830.15566118560567624No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAAAAAAAAAAAAAAAAAA106120.14771318086805296No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACAAAAAAAAAAAAAAAA103610.14421939945098913No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAA99210.13809484238521988No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAAAAAAAAAA87550.12186476616093135No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAA87390.1216420549949034No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCAAAAAAAAAAAAAAAAAA84140.1171182344349602No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAAAAAAAAAA78660.10949037699850211No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAA75610.1052449453960939No Hit
ATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGAAAAAAAAAAAAAAAAA75580.10520318705246365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)