FastQCFastQC Report
Tue 27 Jun 2023
SRR1301054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6966050
Total Bases696.6 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAA3920975.628684835739048No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA2093983.005978998140984No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA1053671.5125788646363434No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAA954531.3702600469419541No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAA901291.2938322291686106No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAAAAAAAAAAAAAAAAAA677050.9719281371796068No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA629150.9031660697238751No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAAA589890.8468070140179872No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAAA565110.8112344872632268No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAAAAAAAAAAAAAAAAAA516450.7413814141443142No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAA492730.7073305531829372No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA445730.6398604661178142No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA391510.5620258252524746No Hit
AGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAAAAAAAA383780.5509291492309127No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAAAAAAAAAAA366650.5263384557963264No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAAAAAAAAAA290560.41710869143919443No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCAAAAAAAAAAAAAAAA290100.4164483459062166No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA220180.31607582489359104No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAA208770.29969638460820697No Hit
CTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAAAAAA171280.2458782236705163No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA168050.24124144960199828No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAG139290.19995549845321237No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAA136690.19622311065812045No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAAAAAAAAAAAAAAAA136320.1956919631642035No Hit
ATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAA134150.19257685488906914No Hit
AGCTGCATCCGGGGTTCGAATCCCCGCCTCACCGCCAAAAAAAAAAAAAA132390.1900503154585454No Hit
AGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAAAAA130270.1870069838717781No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAAAAAAAAAAAAAAAAA120920.1735847431471207No Hit
AGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAAA118130.16957960393623359No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAAA116000.16652191701179292No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAA114750.1647274998026141No Hit
ATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCAAAAAAAAAAAA109710.15749240961520516No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA107390.1541619712749693No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGGAAAAAAAAAA106910.15347291506664468No Hit
TAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAA104590.1501424767264088No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGAAAAAAAAAAAAA100690.14454389503377094No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAA99900.14340982335756994No Hit
TTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAAAA97670.14020858305639494No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCAAAAAAAAA96460.13847158719790986No Hit
TTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAAAAAAA92820.1332462442847812No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGAAAAAAAAAAAA91630.13153795910164298No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAA90730.13024597871103424No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAA90070.1292985264245878No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA88620.12721700246194043No Hit
TGCATCCGGGGTTCGAATCCCCGCCTCACCGCCAAAAAAAAAAAAAAAAA88200.12661407827965632No Hit
GCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAAAA87010.1249057930965181No Hit
CGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAAA86480.1241449601998263No Hit
TTTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAA84560.12138873536652767No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAAA84370.12111598395073249No Hit
TCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAAAAA82510.11844589114347442No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAA80650.11577579833621636No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGAAAAAAAAAAAAAAAAAA80600.11570402164784921No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA80570.11566095563482891No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA79120.11357943167218151No Hit
ACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAAAAAAAAAAAAAA78810.11313441620430516No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAA77480.11122515629373893No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAAAAAAAAAAAAAAA76050.10917234300643835No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAA75220.10798084997954364No Hit
TGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAAAAAAAA74480.1069185549917098No Hit
TTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAAAAAAAAAAA74050.10630127547175228No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAA73620.10568399595179477No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAAAAAAAAAA72720.10439201556118605No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAAAAAAAAAAAAAAAA70600.10134868397441879No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACAAAAAAAAAAAAAAAA70270.10087495783119559No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA69990.10047300837633955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)