FastQCFastQC Report
Tue 27 Jun 2023
SRR1301056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150616
Total Bases415 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAA3160287.614002355313043No Hit
TCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGT1115942.688612967328223No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCTAGCCATG887772.138887336241175No Hit
ACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACG869732.0954239081620654No Hit
TCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACC624481.5045477586941312No Hit
AACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCA497141.1977499243485785No Hit
TTCTGGCGCCGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCAAAA489871.1802344519464099No Hit
AGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAAC449541.083068151811683No Hit
TTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC448141.0796951585017742No Hit
TACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACAC442951.0671909904457555No Hit
AGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTC371130.8941564336474392No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA338940.8166016803288958No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAAC321620.774872934523454No Hit
AACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACAC311930.7515270022570144No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATACG297630.7170742848772327No Hit
ATCAGGTAACATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGA253020.6095962623379277No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC235170.5665905976365917No Hit
TTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAG225010.5421123033303973No Hit
ACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTG224880.5417990968087628No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC215020.518043586783263No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTC203160.48946951488646506No Hit
AGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCT196540.4735200750924682No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCT189430.45639008764000333No Hit
ACCAACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACA186430.4491622448330561No Hit
TAACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCC184860.44537967376408705No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTCG169760.40899953163578606No Hit
GGTGAGGTGGCCGAGAGGCTGAAGGCGCTCCCCTGCTAAGGGAGTATGCG167380.4032654430089413No Hit
AATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGC164920.3973386119072446No Hit
ACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGA159800.38500309351672135No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAA157510.379485840174085No Hit
AGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCT156050.3759682900080374No Hit
AAGTTGCAGGGTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTT147050.35428476158719574No Hit
TAACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACA138560.33382996644353513No Hit
TTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCA132140.31836238283666807No Hit
TAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAA129240.3113754681232858No Hit
AAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTG126590.3049908736438157No Hit
AAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGC125090.30137695224034217No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCC122360.2947996152860202No Hit
AGGTTCTGGCGCCGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCA121830.2935226963901262No Hit
ACTAACTGGAGGACCGAACCGACTAATGTTGAAAAATTAGCGGATGACTT117830.2838855726475299No Hit
TGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTA115460.2781755768300416No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA110900.26718925576348185No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGT110010.2650449957307542No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAA105330.2537695609519165No Hit
AGGGAGTATGCGGTCAAAAGCTGCATCCGGGGTTCGAATCCCCGCCTCAC104200.25104707349463307No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAAA99210.2390247616257442No Hit
ACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGC99160.23890429757896176No Hit
AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTG91960.22155747484228847No Hit
TAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCC90230.2173894188236156No Hit
TCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTA89640.21596794307158262No Hit
TCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAAC89420.21543790126573983No Hit
TTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTG89380.21534153002831388No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTC88100.21225765043068304No Hit
TAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGC86750.2090051211675568No Hit
TTTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC84650.20394563120269377No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCA84090.20259643387873028No Hit
AACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACT83800.20189774240739206No Hit
TTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCTAGCCAT81080.19534449826242659No Hit
TTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGCAACTGTT78490.1891044606390955No Hit
TTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT77920.18773117050577554No Hit
AGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGC77400.18647834441923802No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTC72770.17532337368718282No Hit
CACCCGATGCAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACAC71070.1712275960965794No Hit
AAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTG68530.16510802252003076No Hit
ACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCA64470.15532634192129555No Hit
AAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGTTTAAGCGTGTAGGCTG63520.1530375250324289No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAAC63070.15195334861138685No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGG61320.14773710697400097No Hit
TTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCT61300.147688921355288No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT60990.14694204426523677No Hit
TCCCGACTTACCAACCCGATGCAAACTGCGAATACCGGAGAATGTTATCA60130.14487006266057859No Hit
AACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCT59550.14347267971790212No Hit
TGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCA56990.1373049205226405No Hit
TCCAAAGGCGATCTGGGCCTGGTAATCGCGTGCCTGCCGTACGCATAAGC56370.13581116634253806No Hit
ATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCC54470.13123353256480486No Hit
TTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGT53160.12807737453910456No Hit
TTACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCG51840.12489712370404779No Hit
TCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACG50970.12280104929003309No Hit
AGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGC50820.12243965714968574No Hit
CTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC50760.1222951002935468No Hit
TACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACT49920.12027130430760156No Hit
ACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTG49700.11974126250175877No Hit
AGGGGGGACGGAGAAGGCTATGTTGGCCGGGCGACGGTTGTCCCGGTTTA49240.11863299327136019No Hit
AAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCC48290.11634417638249359No Hit
TGGTGAGGTGTCCGAGTGGCTGAAGGAGCACGCCTGGAAAGTGTGTATAC47870.11533227838952098No Hit
TAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGAC47760.11506725748659959No Hit
CAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTC45620.10991139628431057No Hit
TAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTT45190.10887540548198146No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAA45180.10885131267262499No Hit
TATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTG43670.10521329845979488No Hit
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT42500.10239443976508547No Hit
ATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGC42070.10135844896275636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)