FastQCFastQC Report
Tue 27 Jun 2023
SRR1301057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6768833
Total Bases676.8 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAAA1248551.8445572523358162No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACAAAAAAAAAAAAAAAA866291.2798217949829758No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAA654970.9676261772154816No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAAAAAAAAAAAAAAAAAA600460.8870953087481993No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAAAAAAAAAAAAAAAAAA588210.8689976543962601No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAA539230.7966365841792817No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAAAAAAAAAA470250.6947283231836271No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAA430750.6363726213957414No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACAAAAAAAAAAAAAAA315690.4663876328460164No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA303470.44833429928024515No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCAAAAAAAAAAAAAAAA296080.43741661228752426No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAA260850.385369235730886No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAAAAAAAAAAAAAAAAA244180.3607416522168592No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGAAAAAAAAAAAAAAAAA225010.332420669855498No Hit
ATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCAAAAAAAAAAAA217900.32191664353367855No Hit
TATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCAAAAAAAAAAA214420.31677543233818883No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAAAAAAAAAAAAAAAAAA210510.31099895654095766No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGAAAAAAAAAAAAAAAAAAA210130.3104375599161628No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA208100.3074385200521272No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAA179400.2650383012847266No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAAA177240.2618472046806296No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCAAAAAAAAAAAAAAAAAA172180.2543717654136245No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAA165570.24460641886127196No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA163550.24162215259262565No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGAAAAAAAAAAAAAAAAAA144350.21325684944509637No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAAAAAAAAAAAAAAAAAAA144270.21313866068198165No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCAAAAAAAAAAAAAAAAAAAA132040.1950705535208211No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAAAAAAAAAA129780.19173172096283067No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA123100.18186295924275278No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAA123080.1818334120519741No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAAAAAAAAAAA119150.1760273890639642No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA116640.1723192166212403No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAAAAAAAAAAA112690.16648364644245175No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAA106820.15781154594891025No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAAAAAA98370.1453278578449195No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCAAAAAAAAAAA81800.12084801028478616No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTAAAAAAAAAAAAAAA81310.12012410411070859No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCAAAAAAAAAAAAAAAAAAA79780.11786374401613986No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGACAAAAAAAAAAAAAAAAA75560.11162928676183914No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTAAAAAAAAAAAAAAAAAAA75340.11130426766327371No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCGAAAAAAAAAAA74500.1100632856505693No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAG74350.10984168171972923No Hit
CACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAA74310.1097825873381719No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAAAAAAAAAAAAAAAAAAAAA73500.10858592611163549No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTAAAAAAAAAAAAAAAAAAA71180.10515845198130903No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAA69960.10335607334380978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)