FastQCFastQC Report
Tue 27 Jun 2023
SRR1301059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1301059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6290309
Total Bases629 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAAA2339443.719117773069654No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAA738611.1742030478947854No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAAA548120.8713721376803587No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAAAAAAAAAAAAAAAAAA432990.6883445630413386No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCAAAAAAAAAAAAAAAAAA432050.68685020084069No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCAAAAAAAAAAAA336830.5354744894090259No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCAAAAAAAAAAAAAAAA308360.49021439169363545No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAA282430.44899225141404026No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACAAAAAAAAAAAAAAAA254450.4045111297394134No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAAAAAAAAA243750.38750083660437035No Hit
ATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCAAAAAAAAAAAA197890.31459503817697987No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAAAAAAAAAAAAAAAAAA171040.27191033063717535No Hit
GCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAAAA167650.2665210882327085No Hit
TGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAAAAAAAAA167010.2655036501386498No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAAAAAAAAAAAAAAAAAA162560.2584292758908982No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAAAAAAAAAAAAAAAAA154480.2455841199534077No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAAAAAAAAAAAAAAAAAA149540.23773076966489248No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAAAAAAAA116440.18511014323779643No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCAAAAAAAAAAA104150.16557215233782632No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTAAAAAAAAAAAAAAA101650.1615977847829097No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAAAAAAAAAAAAAAAAAAA100120.15916547183930074No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAAAAAAAAAAAAAAAAAAA96220.1529654584536308No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAAAAAAAAAA95220.15137571143166417No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGAAAAAAAAAAAAAAAAAA85630.13613003749100402No Hit
CGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCAAAAAAAAAAAAAAA83990.1335228523749787No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAAAAAAAAAAAAAAAAA82940.13185361800191372No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAAAAAAAAAAAAAAAAA81680.12985053675423575No Hit
AGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAAAAAAAAAAAAAAA80320.12768848080436113No Hit
ATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAA77800.12368231830900518No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTAAAAAAAAAAAAAAAAA76650.12185410923374353No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCAAAAAAAAAAAAAAAAAAAA73350.11660794406125358No Hit
TTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAAAA71810.11415973364742496No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCAAAAAAAAAAAAAAAAAA70960.1128084486787533No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAAAAAAAAAAAA68800.10937459511130534No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCAAAAAAAAAAAAAAAAAAAA66060.10501868827111673No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGAAAAAAAAAAAAAAAAAAAA64510.10255458038706843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)