FastQCFastQC Report
Fri 29 Sept 2023
SRR13120321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13120321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24334065
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC7669093.151586058473995No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5546072.2791383190601326No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC4621671.899259330489994No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3372831.386052844027498No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA3263121.340967898294017No Hit
GTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACG3074261.2633565333206762No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2034570.8360995172816379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTG1949210.8010211199813924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1947570.800347167643384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCA1908680.7843654564085367No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT1693500.6959379783032551No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1667980.6854506224093673No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1626310.6683264797722863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT1490120.6123596694592539Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGATCGGAAGAGCAC1419270.583244106564193No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1319180.5421124666182983No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1303490.5356647152869856No Hit
AAGGAGGGGTGAACCGGCCCAGGTCGGAAACAGATCGGAAGAGCACACGT1296360.532734666402839No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1231570.5061094395860288No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1220030.5013671164271156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC1210060.49726997934788125No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACAGATCGGAAGAGCACAC1090930.44831391713632723No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATAGATCGGAAGAGCACACGTC1082180.44471813484512346No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTC1063120.4368854936485129No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1043520.4288309413162166No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC999750.4108438109292467No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG993910.40844388309146046No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA900880.37021352577138267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA811990.33368448715822857No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT797560.3277545284768492No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC783910.32214510810257146No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC777070.31933423371721903No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC765560.3146042389547328No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC750480.3084071650174354No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC742000.30492233829407456No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT723860.2974677679212248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCAGATCGGAAGAGCACAC687620.28257506503742796No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCAGATCGGAAGAGCAC685130.28155180813398833No Hit
AAGAGGTAAACGGGTGGGGTCCGCGCAGATCGGAAGAGCACACGTCTGAA653460.2685371309725687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTAGATCGGAAGAGCACA647100.26592351093004807No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG645560.26529065324679624No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTC624500.25663611895505334No Hit
GGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA614940.25270746996032106No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT608430.2500322079356655No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG587930.24160780371055968No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA565580.23242314837245648No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC558590.22955063200496917Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAGGTGGTTTTCCCAGGGCGAGGCTTAGATCGGAAGAGCACACGTCT541270.22243303780112364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT509910.20954575406944956No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCAGATCGGAAGAGCACACGTCTG489090.20098984694912253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA454450.18675465854143153No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG439180.1804795047600966No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAG428690.17616867547612783No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCAGATCGGAAGAGCACACGTCTG428030.17589745075473415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGC421610.17325917391935955No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA421560.17323862659198125No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGT420010.17260165944325373No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT418020.1717838758135971Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC414600.17037843862092092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC412450.16949490354365374Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA408420.1678387889569622No Hit
ACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCAGATCGGAAGAGCAC404120.1660717188024278No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCAGATCGGAAGAGCACACGTCTGA400060.16440327581930927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC396010.1627389423016664No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA391590.1609225585614241No Hit
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGA382520.15719527337499922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG374620.1539487956492267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGGCTCGTGCGTCGATGAAGAACGCAGCAGATCGGAAGAGCACACGT346300.14231078942215367No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC341840.14047796782000868No Hit
AAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGATCGGAAGAGCA338260.13900677917972193No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG337000.13848898652978858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA335300.13779037739892616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACA328720.13508634911594097No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG327030.13439184945055418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACG322590.13256724677936055No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG322520.1325384805210309TruSeq Adapter, Index 5 (100% over 49bp)
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTAGATCGGAAGAGC316970.13025772718203882No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA316700.130146771614196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG312320.1283468257358563No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG310670.12766876393237217No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC309110.127027687318169No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG306820.12608661972424254No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGT303540.12473871504822559No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA303310.12464419734228539Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCAGATCGGAAGAGCACAC303090.12455378910182083No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC298560.12269220124134622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT292660.12026761661070601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACGGCTGGGAAGGCCCGGCGGGGAAGGTAGATCGGAAGAGCACACGT286040.11754715046581818No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG282780.11620746472075258No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG277030.11384452207224727No Hit
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACA269260.11065146739765838No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCAGATCGGAAGAGCACACGTCTG264380.10864604824553563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAGATCGGAAGAGCACAC264200.10857207786697373No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCA261400.10742142753378853No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG259410.10660364390413192No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT259380.10659131550770494No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCT258230.10611872697800387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCT254790.10470507085437637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA247870.1018613207452187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)