FastQCFastQC Report
Fri 29 Sept 2023
SRR13120322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13120322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14425407
Total Bases721.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA6893724.778873830041676No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5641843.9110438963697867No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3706462.569397175414184No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3072572.1299710989090292No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2821061.9556189991727788No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG2724881.8889449704954597No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2141421.4844780462693357No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1695661.1754677008419936No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1581751.0965028577703215No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1462151.013593585262447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG1269350.8799405105173117No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1266690.8780965417474875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1134200.7862516461407293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1131890.784650304840619Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1098360.7614065932420486No Hit
GCCGCCTGGATACCGCAGCTAGGAATAGATCGGAAGAGCACACGTCTGAA862880.598166831618685Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC833460.5777722597358951No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT814540.5646565119445157No Hit
TGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGAA716600.49676241370520774Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA687830.47681843569474336No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG678520.47036454500035946No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC675260.468104643425312No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC644570.44682968043813254No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG613930.425589378518055No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG602900.41794314711536384No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG594520.4121339522690764No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG589360.4085569301441547No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT588950.4082727093939187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC575810.3991637809595251Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG560530.3885713588531679No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC557600.3865402203209934No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACGTCT550600.3816876709267198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT534760.3707070448688207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG496860.3444339560055394No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA494230.3426107838759766No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG474050.3286215771936279Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT454560.31511069323728613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC449210.31140195905737705No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGATCGGAAGAGCACACGTC424500.29427245969559124No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA409610.2839503939126293No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT371650.25763571176882566No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCT370920.25712966018913713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG369710.2562908623652699No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA366560.25410721513784673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC354260.24558059263076595Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT351370.243577182952273Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC351290.2435217252449099No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCT346430.24015266952259995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT345830.2397367367173765No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG338970.23498123831098838No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT336270.23310954068748285No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT307770.21335273243936895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACG297600.20630267139083147No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA286100.19833062595738202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC285610.19799094749978285No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA281330.19502396015585557No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTAGATCGGAAGAGCACACGTCT280830.194677349484836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT279190.19354046648389192No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC279060.19345034770942685No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC274150.19004663092001495Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC268460.18610220148381254No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG265840.18428596156767016No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT254620.17650801810999164No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA254150.17618220407923324No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT253870.1759881021034623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC250970.17397776021154898No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC247590.17163467207545688No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC246560.1709206540931566No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC245950.17049778907451277No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAGATCGGAAGAGCACAC239180.16580468058890815No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT234380.16247721814712057No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT233750.16204048870163595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTC230480.15977365491316814No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGT226670.1571324815999992No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC225280.1561689039345649No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC220960.15317418773695607No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGT219960.15248096639491698No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT218730.1516283041442089No Hit
AGCTACCATCTGTGGGATTATGACTGAACAGATCGGAAGAGCACACGTCT205250.14228368045352205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG203300.1409318988365458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA200370.13890076030437132Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA199040.1379787759194593No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCAGATCGGAAGAGCAC198800.13781240279736995No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGTCTGA191070.13245380182340782Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTG190460.132030936804764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA190270.13189922474977656No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGT189040.1310465624990685No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACAC186710.12943135677211742No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGA184100.1276220490693954Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGCACACGTC183360.1271090652762865No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC182210.12631186073294154No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG181110.12554931725669854No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT176630.12244368564436345No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA175670.12177819315600594No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT171470.11886666351944178No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGT168220.11661369415781477No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC168120.11654437202361083No Hit
GCTGAGAAGACGGTCGAACTTGACTATCAGATCGGAAGAGCACACGTCTG167630.11620469356601171Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC163490.11333475720996988Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC160900.11153931393408865No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT160280.11110951670202443No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA158740.11004195583528423No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATAGATCGGAAGAGCACACGTCT157480.10916849694431498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC156040.10817025821177871No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC156030.1081633259983583No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG154920.10739385030869493No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT152640.10581330564884582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA152280.10556374596571173No Hit
CGCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTG149930.1039346758119199Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA149100.10335930209802746No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA145990.10120338372428592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)