FastQCFastQC Report
Fri 29 Sept 2023
SRR13120323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13120323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18915948
Total Bases945.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC7262783.8395009332865584No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC6680173.531501566826045No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA5048572.6689489736385403No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA4361252.3055942001955176No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2913931.5404620482145541No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2741291.4491951447529883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2426881.282980900560733No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2259911.1947114678048385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1779410.94069300676868No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1677550.8868442649556871Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1622430.8577048319227775No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1574850.8325514534085208No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1529940.8088095822636011No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1250660.6611669687398167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1193930.6311764020497412No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC1088600.5754932293110554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1079550.5707089065797812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC989050.5228656792670396No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG936860.4952752037592829No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC872190.46108712077237685No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG821260.4341627498658803No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC775790.41012483223151175No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT731270.38658913632031555No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG697180.36856730627510714No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA638380.3374824248829612Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG637830.33719166493796665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC635230.33581716337981055No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC614240.32472070656992713Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT606580.3206712135178211Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCCAAGAATGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGT588010.3108541004659138No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC586500.3100558322532923No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT584450.30897209064013076Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT571060.3018934076156268Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA565590.29900166779904447No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC523530.2767664618236422No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA486430.2571533818976453No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG466280.24650099482193544No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG461820.24414319599525225No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC455610.24086025188904095No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA455380.24073866136658864No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC447360.2364988527141225No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG430980.22783949289773897No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG430670.22767561001965111No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA428790.22668173966221516No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT386940.2045575511203562No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA379080.2004023271791612Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA367610.19433866068991096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA348490.1842307876930091No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG346310.18307832100193974No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT333260.17617938048888696No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC332120.17557671442108003No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA311160.16449611724455998No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA296620.15680948160779465No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG289670.15313533321195427No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC276520.14618352725435702No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA271610.14358783392722374No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC270770.14314376419305022Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC267190.14125118127835834No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG263030.13905197878530856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG262150.13858676287331725No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC254180.13437338694312334No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT253410.13396632302013095No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGT250730.13254952910633927No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACA249610.13195743612744126No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC236720.1251430803256596No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC235350.1244188237354004No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC234190.1238055845786846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT232970.12316062615524213No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT231710.12249452155398186No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC231590.12243108302052849No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC226950.11997812639366527No Hit
TCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAA223470.11813840892351786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT221480.11708638657708301No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT220970.11681677280990622No Hit
CATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACG215860.11411534859368402No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC214960.11363955959278382No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCT213660.11295230881370577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG210510.1112870473105551No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAGATCGGAAGAGCACAC206810.10933102586240986No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA206470.10915128335095867No Hit
GCCAAGAATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG201190.10635998787901088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA197830.10458370894231683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTC197270.10428766245286782No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC194470.10280743000562276No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT193720.10241093917153928No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA193440.10226291592681476No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC193310.10219419084890698No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC191060.10100471834665649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)