FastQCFastQC Report
Wed 6 Sept 2023
SRR13142690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9794324
Total Bases489.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1999672.0416620891855324No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA1064211.0865578880175906No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC954100.9741356320252423No Hit
TTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG918940.9382372892708062No Hit
TATCCTGAGTACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT823990.8412933858426573TruSeq Adapter, Index 6 (95% over 21bp)
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT816180.8333193796733701No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT807130.824079334112288No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC732570.7479536106830854Illumina PCR Primer Index 6 (95% over 23bp)
TCCTTGGGAGTCAGACTGCTGTAGGCACCATCAATATCTCGTATGCCGTC698960.7136378171683926No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT681190.6954946558843673No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC656040.6698165182201447No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCAA595210.6077091180565397No Hit
AGAAGGCAGACCCGGGGAACCTGTAGGCACCATCAATATCTCGTATGCCG569710.5816736305639879No Hit
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT549880.5614272102903682Illumina PCR Primer Index 6 (95% over 24bp)
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT519380.5302867252502572No Hit
TATCCTTGGGAGTCAGACTGCTGTAGGCACCATCAATATCTCGTATGCCG486900.49712466118131277No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA476520.4865266862725799No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT433780.44288916723604405Illumina PCR Primer Index 6 (95% over 21bp)
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG429490.4385090793402383No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC409010.4175990093854359No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG389270.3974444790676723No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA371190.3789848079356983No Hit
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC369700.3774635186665256No Hit
TAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTGTAGGCACCATCAAT364910.37257293101596395No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA360670.36824389309563377Illumina PCR Primer Index 6 (96% over 29bp)
GTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG343920.35114215131131055Illumina PCR Primer Index 6 (96% over 25bp)
TCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTATG331880.338849317216788No Hit
TATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATA320090.3268117329996435No Hit
ATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATGC314320.3209205658297602No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC305830.3122522799940047No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA287940.293986598768838No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCA286600.2926184594260921No Hit
TAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTC285700.29169955986753143No Hit
TTAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTGTAGGCACCATCAA281830.28774829176572064No Hit
TACGGTCCTAAGGTAGCGAAACTGTAGGCACCATCAATATCTCGTATGCC281610.28752367187362804No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTGTAGGCACCATCAATATCT247780.2529832584668426No Hit
AACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCCTGTAGGCACCATCA247580.25277905856494026No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC244480.24961396008545358No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT241200.24626508169425476No Hit
TATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATG239820.24485610237112843No Hit
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG215340.2198620343782787No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGCTGTAGGC213680.21816717519248904No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA205550.20986644918015782No Hit
AAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT203530.2078040301709439No Hit
GGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCAA202520.20677282066633695No Hit
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATC202410.20666051072029068No Hit
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC202220.20646652081348338Illumina PCR Primer Index 6 (95% over 23bp)
CAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT199930.20412843193670127No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC189500.19347940705249284No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC179510.1832796219524696No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA165370.16884268888797227No Hit
AGAAGGCAGACCCGGGGAACTCTGTAGGCACCATCAATATCTCGTATGCC164150.16759706948636782No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC157010.1603071329884533No Hit
TAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT153220.15643754484740346TruSeq Adapter, Index 6 (95% over 21bp)
TCCGCTCAGGGTTAGTCGGGCTGTAGGCACCATCAATATCTCGTATGCCG152740.15594746508283777No Hit
AGAGCCGCAGTGAATAGGCCCCTGTAGGCACCATCAATATCTCGTATGCC150460.1536195862011508No Hit
TTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATGCCGTC150000.15314992642677536No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA149220.15235354680935612No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCCTGTAGGCACCATCAATATCTC148990.1521187169221684No Hit
ACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGCTGTAGGCACCA143610.14662573956099473No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATATCTCGTA138200.14110213221453569No Hit
TTTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATGC137480.14036701256768716No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC137330.1402138626412604RNA PCR Primer, Index 6 (95% over 23bp)
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT129720.13244405637387532No Hit
TTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATGCC128770.1314741068398391No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA128420.13111675701150993No Hit
TAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATC125160.12778829861050137No Hit
CTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG124490.12710422893912843No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGCTGTAGGCACCATCAAT123400.1259913394737605No Hit
AGAGCCGCAGTGAATAGGCCCAGCTGTAGGCACCATCAATATCTCGTATG117200.11966114251478713No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT116160.11859930302489483No Hit
AGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT115940.11837468313280224Illumina PCR Primer Index 6 (100% over 21bp)
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC113620.11600596427073476No Hit
TTATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAAT113430.11581197436392751No Hit
ACGGTCCTAAGGTAGCGAAACTGTAGGCACCATCAATATCTCGTATGCCG112670.11503601473669851No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC112320.11467866490836938No Hit
ATGCCTAAGGTTTCCTGAGGCTGTAGGCACCATCAATATCTCGTATGCCG111630.11397417524680621No Hit
TGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGC110540.11286128578143831No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG110520.11284086579124808No Hit
TCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGCCG109570.11187091625721184No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT109330.11162587637492899TruSeq Adapter, Index 6 (95% over 22bp)
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCA106890.10913463757172011No Hit
CATCCTGAGTACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT106620.10885896770415193TruSeq Adapter, Index 6 (95% over 21bp)
AGGTGAGAGGTGCCCGTACCGCAAACCCTGTAGGCACCATCAATATCTCG105390.10760313830745236No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT105260.10747040837121583No Hit
AGCGAAGTTCCTGATTCCACACTGCCCTGTAGGCACCATCAATATCTCGT105080.10728662845950368No Hit
AAAATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTA104780.10698032860665015No Hit
AAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC103460.10563260925409451No Hit
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCACTGTAGGCACCATCAATA103410.10558155927861891No Hit
AGAAGGCAGACCCGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT102940.10510168950914836TruSeq Adapter, Index 6 (95% over 21bp)
CAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC99510.10159966119152276No Hit
GGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCAAT99130.10121168137790827No Hit
TCCGCGTCCAAGCAGTTAGGCTGGGAAATAGGCAAATCCGTTTCCTGTAG98790.10086454154467424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)