FastQCFastQC Report
Wed 6 Sept 2023
SRR13142691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25441007
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT4362041.714570496364393No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT3971901.5612196482631366No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT3314941.3029908761080096No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG3165761.2443532600733924Illumina PCR Primer Index 6 (96% over 29bp)
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC2226940.875334848184272No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2050680.806052999395818Illumina PCR Primer Index 6 (96% over 29bp)
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC1977530.7773002067095851No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1777360.6986201450280644No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTG1078930.42409091747036587No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG1076120.42298640144236427No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC959160.37701337844056254Illumina PCR Primer Index 6 (95% over 23bp)
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC877010.3447229899351075No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT858990.3376399369726206No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT827870.32540771676215485Illumina PCR Primer Index 6 (95% over 21bp)
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA820470.32249902686635007No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT809580.31821853592509136TruSeq Adapter, Index 6 (95% over 22bp)
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT791510.3111158296524977Illumina PCR Primer Index 6 (95% over 24bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC753100.296018156828462No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT751110.2952359550862118No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA678650.26675437807945257No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC584060.2295742460194284No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT567260.22297073382354718No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT522250.20527882406541537No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC501210.19700871117247837No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTG494600.19441054357636078No Hit
ATGCCTAAGGTTTCCTGAGGCTGTAGGCACCATCAATATCTCGTATGCCG471830.1854604261537289No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC457330.17976096622275997No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT450210.17696233486355317No Hit
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT435150.1710427578593882RNA PCR Primer, Index 6 (95% over 22bp)
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT433520.17040205994990684No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG412240.16203761116845727No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT405260.15929400907754948No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC398880.15678624670792315No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT397100.15608658886812146No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC394880.15521398189938No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA387850.1524507264983654No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA379400.14912931709031801No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC368770.14495102336161458TruSeq Adapter, Index 6 (95% over 23bp)
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT346830.1363271508867554No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT346280.1361109644755807No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG330670.12997520106024105No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC321310.12629610140825007No Hit
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC321040.12618997353367342No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGCTGTAGGCACCATCAAT315630.1240634853801188No Hit
GTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT313110.12307295855073662No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA307740.12096219304526742No Hit
TATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC306610.120518028236854No Hit
TATCCTGAGTACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT303480.11928773102416897TruSeq Adapter, Index 6 (95% over 21bp)
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA301420.11847801464776925No Hit
CTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC301360.11845443067564111Illumina PCR Primer Index 6 (95% over 23bp)
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA295870.11629649722591563No Hit
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT294580.11578944182516046No Hit
TGGGAGGAGGTGAAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT292960.11515267457770048RNA PCR Primer, Index 6 (95% over 21bp)
GAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT290450.11416607841033964No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA289200.11367474565766993No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG286380.112566298967647No Hit
TCCGCTCAGGGTTAGTCGGGCTGTAGGCACCATCAATATCTCGTATGCCG281700.1107267491416515No Hit
ACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCAAT269830.1060610533222997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)