FastQCFastQC Report
Wed 6 Sept 2023
SRR13142692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11327309
Total Bases566.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT2429812.1450902416452133No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT1477491.3043609916530041No Hit
TTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG1384821.22254985716378No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1304541.1516768898950316No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1180681.0423305305787987No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA1058540.9345026254691207No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC870700.7686733009578886No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC853980.7539125135546315No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC749760.6619047825039468Illumina PCR Primer Index 6 (95% over 23bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA717930.6338045514605455Illumina PCR Primer Index 6 (96% over 29bp)
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA675560.5963993742909283No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC642680.5673721799237577No Hit
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT572390.5053186065640126Illumina PCR Primer Index 6 (95% over 24bp)
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG549490.48510197788371445No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCA526810.4650795700903012No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCAA463530.40921458044448156No Hit
TATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATA409460.36148038338143684No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA395480.3491385288421107No Hit
TCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTATG358350.31635934006920796No Hit
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG353740.31228952966675494No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT308150.2720416649709123Illumina PCR Primer Index 6 (95% over 21bp)
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATC305490.26969335788403054No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA304610.2689164743364907No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC304520.26883702033731044No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT286610.25302567450044844No Hit
GTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG279190.24647513367914656Illumina PCR Primer Index 6 (96% over 25bp)
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC267610.2362520524512927No Hit
AACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCCTGTAGGCACCATCA244920.21622081643574834No Hit
AAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT243170.2146758775627998No Hit
CAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT237690.20983801183493803No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC232180.20497366144068288No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC232070.2048765509972404No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC221090.19518316309725461No Hit
TATCCTGAGTACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT220490.19465346976938652TruSeq Adapter, Index 6 (95% over 21bp)
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT211620.18682283673907016No Hit
GGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCAA201260.17767679861121474No Hit
TACGGTCCTAAGGTAGCGAAACTGTAGGCACCATCAATATCTCGTATGCC201150.17757968816777225No Hit
AGAAGGCAGACCCGGGGAACCTGTAGGCACCATCAATATCTCGTATGCCG197080.17398660176040048No Hit
CTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG188920.16678277250139464No Hit
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC187220.16528197473910175Illumina PCR Primer Index 6 (95% over 23bp)
TTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATGCCGTC182250.16089434833992788No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT178830.15787509637107983No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGCTGTAGGCACCATCAAT178300.1574072005981297No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA175520.15495295484567428No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG174220.1538052859686268No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG155770.1375172161366835No Hit
TCCTTGGGAGTCAGACTGCTGTAGGCACCATCAATATCTCGTATGCCGTC155650.13741127747110987No Hit
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA154370.13628126503832463No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT153270.13531016060389983No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT152700.13480695194242515No Hit
TGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGC149670.1321320006366914No Hit
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT146820.12961595732931802RNA PCR Primer, Index 6 (95% over 22bp)
TTTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTA145290.12826523934325443No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG144550.12761195090555047No Hit
TTTCTGGACGTGGGTTCGACTCCCACCGTCTCCCTGTAGGCACCATCAAT143660.1268262391358795No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA143500.12668498758178134No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG141230.12468098115801379No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT140420.12396589516539189Illumina PCR Primer Index 6 (95% over 22bp)
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC140130.12370987672358899No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT138210.12201485807441113TruSeq Adapter, Index 6 (95% over 22bp)
TTGCGTTGGTTCGAATCCAGCTAGCCCAGCCACTGTAGGCACCATCAATA136420.12043460631293804No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG136280.12031101120310217No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCATC131510.11609994924655095No Hit
TTATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAAT131260.1158792436932726No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATATCTCGTA127300.11238326772934332No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG125250.11057348219246071No Hit
ATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATGC123490.10901971509738102No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTGTAGGCACCATCAATATCT123070.10864892976787338No Hit
TATCCTTGGGAGTCAGACTGCTGTAGGCACCATCAATATCTCGTATGCCG119780.1057444446867301No Hit
GCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATGC118990.10504701513837046No Hit
TTCCCCCTGTGAGAGTAGGACGCCGCCAAGCCTGTAGGCACCATCAATAT117710.10391700270558524No Hit
AAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCA116270.10264573871870185No Hit
TAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATC116250.10262808227443959No Hit
TATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTA115870.10229260983345646No Hit
TGGGAGGAGGTGAAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT115740.10217784294575172RNA PCR Primer, Index 6 (95% over 21bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA114680.10124205139985146Illumina PCR Primer Index 6 (100% over 28bp)
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC114310.10091540718099948No Hit
TCCGCTCAGGGTTAGTCGGGCTGTAGGCACCATCAATATCTCGTATGCCG113810.10047399607444275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)