FastQCFastQC Report
Wed 6 Sept 2023
SRR13142693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22321569
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT3486041.5617360948058803No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG2713341.2155686726143669Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT2711851.2149011568138424No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC2324391.0413201688465537No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT2322561.0405003340042986No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1744710.7816251626397769Illumina PCR Primer Index 6 (96% over 29bp)
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC1591410.7129471947066086No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC1426180.6389246203974281No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1155120.5174905043637389No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC990950.4439428070670122Illumina PCR Primer Index 6 (95% over 23bp)
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG895640.40124419569251607No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC753970.3377764349808922No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA731490.3277054583394205No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTG717630.32149621740299705No Hit
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT714890.3202687051255223Illumina PCR Primer Index 6 (95% over 24bp)
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC701590.31431034261077256No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT667270.2989350793396289TruSeq Adapter, Index 6 (95% over 22bp)
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC587240.2630818648993716No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA547370.2452202172705691No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA546430.24479909991990256No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT546380.24477670006082455No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC469910.2105183555869213RNA PCR Primer, Index 6 (95% over 23bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG461030.20654014061466738No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT426570.19110215773810524No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT424530.19018824348772256No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT404990.18143437856003758Illumina PCR Primer Index 6 (95% over 21bp)
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA391830.17553873565070627No Hit
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT383420.17177107935378558RNA PCR Primer, Index 6 (95% over 22bp)
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC373580.16736278708723387No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT369330.16545879906560332Illumina PCR Primer Index 6 (95% over 22bp)
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA357930.16035163119581783No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGCTGTAGGCACCATCAAT357610.1602082720977186No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC336510.1507555315667998No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA335950.15050465314512615No Hit
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC335590.15034337415976448No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTG328740.14727459346607757No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC326140.14610980079402125No Hit
ATGCCTAAGGTTTCCTGAGGCTGTAGGCACCATCAATATCTCGTATGCCG324050.14517348668456057No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT321490.14402661389976665No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC307820.13790249242783964No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT305490.13685865899480454No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC305060.13666602020673368No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT303820.13611050370159913No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC296580.13286700410710375TruSeq Adapter, Index 6 (95% over 23bp)
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG295350.1323159675737848No Hit
TATCCTGAGTACGGCGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT289120.1295249451326652TruSeq Adapter, Index 6 (95% over 21bp)
GTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT288530.1292606267955447No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT286270.12824815316521881No Hit
TTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG281790.12624112579182942No Hit
AAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC278870.12493297402167383No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT273890.12270194805750437No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC272250.12196723267974575No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC268040.12008116454537761No Hit
TATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC264490.11849077455083913No Hit
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC263450.11802485748201662Illumina PCR Primer Index 6 (95% over 23bp)
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA261020.11693622433082548No Hit
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA259820.11639862771295334No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA250630.11228153361441573No Hit
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT245570.11001466787572146No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC245260.10987578874943782No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG241120.10802108041777887No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCA238450.10682492794301332No Hit
TGGGAGGAGGTGAAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT232130.1039935857555533RNA PCR Primer, Index 6 (95% over 21bp)
CAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC230100.10308415147698624No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT225750.10113536373719965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)