FastQCFastQC Report
Wed 6 Sept 2023
SRR13142694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215751
Total Bases560.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1946451.7354611385363317No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1625131.4489711834722436No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1277981.1394511165592032Illumina PCR Primer Index 6 (96% over 29bp)
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA946550.8439470526761873No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT862080.7686333264709603No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG626820.558874746773533No Hit
TCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGCCG542290.48350752437353506No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT472960.42169267131554544No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA397890.3547600156244553No Hit
TATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGC381860.34046761558811356No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC351330.3132469684820927No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT338130.30147780563245385No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA329460.29374760548803197No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT312640.27875083888720426TruSeq Adapter, Index 6 (95% over 22bp)
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC304380.2713861960737181No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG274130.24441519787662905No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC261990.23359113446794602No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT242760.21644560404381302Illumina PCR Primer Index 6 (95% over 21bp)
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT217260.1937097212661016No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT215970.19255955307852324No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT212660.18960834633365167No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC204670.18248443639663542No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGTA193090.17215967080581585No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT186830.16657823448469922No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC182940.16310989785704053No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC181280.1616298364683738No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC180570.16099679816358264No Hit
TAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT179110.15969505742415285TruSeq Adapter, Index 6 (95% over 21bp)
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC175640.15660119415989174No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG171020.15248198716251815No Hit
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT164630.1467846424193975No Hit
TGGGAGGAGGTGAAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT145250.1295053715083368RNA PCR Primer, Index 6 (95% over 21bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA143330.12779349327566206Illumina PCR Primer Index 6 (100% over 28bp)
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG137030.12217639282469804No Hit
AGAAAGGAGGGAAATGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT136380.12159685071467796Illumina PCR Primer Index 6 (95% over 22bp)
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATATCTCGTA131500.11724582687329631No Hit
CTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT121080.10795532104805108No Hit
GGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTATG116580.10394310644021965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)