FastQCFastQC Report
Wed 6 Sept 2023
SRR13142695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505024
Total Bases525.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1097531.0447667706423136No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT932910.8880607983380143No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT890820.8479942549393509No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC610590.5812361780420492No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA534230.50854714848819No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT375310.3572671514125051No Hit
TCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGCCG361560.3441781760803212No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA357290.3401134542862539Illumina PCR Primer Index 6 (96% over 29bp)
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC348660.3318983374050359No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG342050.3256061099908006No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGTA324060.30848097062891056No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA303080.2885095740856946No Hit
TATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGC266580.2537642941129882No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT256930.24457821324349185No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC232600.22141786634661664No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA230490.21940930358655056No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC221780.21111803266703624No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC206780.196839150486472No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT198570.18902384230630984No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT196130.18670114413827135No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG195120.18573969940478002No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG180060.17140370169549352No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC179590.1709562967205025No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT171990.16372166308234992TruSeq Adapter, Index 6 (95% over 22bp)
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT163840.15596347043091No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG151390.14411199822104165No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT149500.14231285906629057No Hit
TAACCCGTGGAGGTTCAAGTCCTCTCGGCCGCACCACTGTAGGCACCATC144110.13718198073607446No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTCACCCTGTAGGCACCATCA135680.12915724895059735No Hit
AGAAAGGAGGGAAATGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT134610.12813868868838377Illumina PCR Primer Index 6 (95% over 22bp)
GGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTATG131840.1255018551123729No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT127740.12159896064968534No Hit
CTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT127470.12134194077043517No Hit
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG125020.11900972334760967No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT108070.10287458648357206Illumina PCR Primer Index 6 (95% over 21bp)
GCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGTAT107580.10240814299900694No Hit
TCCGCTCAGGGTTAGTCGGGCTGTAGGCACCATCAATATCTCGTATGCCG107550.10237958523464583No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT106660.10153237155859901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)