FastQCFastQC Report
Wed 6 Sept 2023
SRR13142696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12102237
Total Bases605.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTATG3183512.6305136810657404No Hit
TTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG2189001.8087565133619512No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1799451.4868738729872832No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1142660.9441725525619769No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1068320.8827458923503151No Hit
TATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATA793280.655482122850511No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA776970.6420052755536022Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTAT775150.640501421348797No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG745350.6158778744789083No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC710510.5870898082726359No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA685790.5666638324798962No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC685190.5661680563684218Illumina PCR Primer Index 6 (95% over 23bp)
TCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGCC661670.5467336327986305No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA644460.5325131213345103No Hit
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT626040.5172927947122503Illumina PCR Primer Index 6 (95% over 24bp)
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCAA592120.4892649185435718No Hit
TATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGTA570800.47164834071585277No Hit
TACGGTCCTAAGGTAGCGAAACTGTAGGCACCATCAATATCTCGTATGCC549760.45426312507348854No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCA506290.4183441457971778No Hit
TGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGC476260.3935305514178908No Hit
ATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATAT454480.3755338785713749No Hit
TCCGCGTCCAAGCAGTTAGGCTGGGAACTGTAGGCACCATCAATATCTCG413090.3413335898148417No Hit
GTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG394070.3256174870811074Illumina PCR Primer Index 6 (96% over 25bp)
TCCGCGTCCAAGCAGTTAGGCTCTGTAGGCACCATCAATATCTCGTATGC323840.2675868932330444No Hit
CTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG317680.262496925155242No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT317240.2621333560068275No Hit
TTATTGGATATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAAT304420.25154027309166066No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT300270.248111154987297No Hit
AACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCCTGTAGGCACCATCA299330.24733443907932062No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA283740.2344525231161809No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC282700.2335931778562922No Hit
TAGGCTGAGCTGTGATGGCGAGCTGTAGGCACCATCAATATCTCGTATGC282410.23335355273574632No Hit
TAGCAATGGGGGGACGCAGGAGGATAGGGTAAGCGCGGCTGTAGGCACCA277180.22903203763072894No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT253110.2091431526254196Illumina PCR Primer Index 6 (95% over 21bp)
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT250420.20692042305897662No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC247490.20449938304794393No Hit
GGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCAA241650.1996738288962611No Hit
ACCGCATGGTTCAAACATAAAAGGTGGCTCTGTAGGCACCATCAATATCT239480.1978807719597625No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA237560.19629428840304483Illumina PCR Primer Index 6 (100% over 28bp)
GCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGCC230740.19065896660262066No Hit
TAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTC225180.18606477463629242No Hit
ACGGTCCTAAGGTAGCGAAACTGTAGGCACCATCAATATCTCGTATGCCG217270.17952879290002335No Hit
TTATCCGCGTCCAAGCAGTTAGGCTGCTGTAGGCACCATCAATATCTCGT212920.17593441609183494No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG211780.17499244148003382No Hit
TCCGCGTCCAAGCAGTTAGGCTGGGAAATAGGCAAATCCGTTTCCTGTAG209300.17294323355260685No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG202190.16706828663163678No Hit
TTTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTAT189750.15678919525373697No Hit
TGGGGACGTTACGGATTCGACAGCTGTAGGCACCATCAATATCTCGTATG186340.1539715343535249No Hit
GGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATCAAT185080.15293040451942894No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCATC182820.15106298116620917No Hit
TGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC181130.14966654511889Illumina PCR Primer Index 6 (95% over 23bp)
AAGGCTGAGCTGTGATGGCGAGCTGTAGGCACCATCAATATCTCGTATGC175650.1451384566340917No Hit
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG173100.14303140816032606No Hit
CCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATGCC171930.14206464474295125No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT160930.13297541603258967No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC160570.13267795036570512No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA156570.12937277628921No Hit
TTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATGCCGTC155370.12838122406626146No Hit
AAGAGTCTTGGAGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGCCG153970.12722441313948818No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT151130.12487773954517666TruSeq Adapter, Index 6 (95% over 22bp)
TTGGGCACTCTAAGGTGACTGCCGGCTGTAGGCACCATCAATATCTCGTA144270.11920936600398754No Hit
TCCTTGGGAGTCAGACTGCTGTAGGCACCATCAATATCTCGTATGCCGTC140630.11620165759437698No Hit
AGAAGGCAGACCCGGGGAACCTGTAGGCACCATCAATATCTCGTATGCCG138860.11473911806552789No Hit
GTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG137870.11392108748159535No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG135510.11197103477646322No Hit
TACGGTCCTAAGGTAGCGAAATCTGTAGGCACCATCAATATCTCGTATGC132670.1096243611821517No Hit
ACTGATTGGACTAGGGGCCCCTGTAGGCACCATCAATATCTCGTATGCCG132390.10939299899679704No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA132150.10919468855220733No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC131300.10849233906095213No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC131250.10845102438499592No Hit
AGCAATGGGGGGACGCAGGAGGATAGGGTAAGCGCGGCTGTAGGCACCAT131000.10824445100521499No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGCTGTAGGC129570.10706285127286798No Hit
ACAACAGGTTGATATTCCTGTACCACCTCCTGTAGGCACCATCAATATCT126120.10421213863189094No Hit
AAGAGTCTTGGAGGTAGAGCACTGATTGGCTGTAGGCACCATCAATATCT125690.10385683241866772No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA124240.10265870681593825No Hit
TTTCTGGACGTGGGTTCGACTCCCACCGTCTCCCTGTAGGCACCATCAAT122580.10128705957419278No Hit
TCCGCGTCCAAGCAGTTAGGCTGGCTGTAGGCACCATCAATATCTCGTAT121510.10040292550873033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)