FastQCFastQC Report
Wed 6 Sept 2023
SRR13142697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065531
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGACTTATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTC2508220.8937012451323297No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1880660.6700959978273705No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1647970.5871864672719002No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC1562430.5567077993286498No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC1451190.5170719912621642No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT1435310.5114138050692859No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1413820.503756725643281No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC1385160.4935449110155799No Hit
ATGACTTATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTC1291620.4602157714386376No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG1274360.4540658788889474No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1148430.4091958922850952No Hit
AAAATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTA1106870.3943876921480659No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT1068250.38062704033641837No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA849910.3028305432738828No Hit
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC845380.3012164637113048No Hit
TAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTC787440.2805719229042914No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC753840.2685999420427855No Hit
TTAGGGCTAGCCTCAAGGTAAGAGTCCTGTAGGCACCATCAATATCTCGT748710.2667720771076806No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA747670.26640151579530064No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC723870.2579213626850673No Hit
AAAATTCCGTCGGAATCCGGGAGGACCTGTAGGCACCATCAATATCTCGT667630.23788254710021342No Hit
TAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCCTGTAGGCACCATCAA603730.21511440492609957No Hit
TGAGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTAT602820.2147901637777671No Hit
TTGACTTATCCTTGGGAGTCAGACTGCCTGTAGGCACCATCAATATCTCG596680.2126024267989086No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA565320.20142857799483643Illumina PCR Primer Index 6 (96% over 29bp)
TTAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCCTGTAGGCACCATCA525580.18726886015447206No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT512690.18267603773468602No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATAT495390.17651189282682733No Hit
AAGAACCTTACCAGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT486410.1733122384180082Illumina PCR Primer Index 6 (95% over 21bp)
TCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGCCG473550.1687301052668485No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA467660.16663144552654285No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT463810.16525965605282864No Hit
TTGGAGCTGACAGATACTAATCGATCGAGGACCTGTAGGCACCATCAATA408760.1456448481234864No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT403720.14384905099426054No Hit
AGGGCTAGCCTCAAGGTAAGAGTCCTGTAGGCACCATCAATATCTCGTAT401580.14308654983224794No Hit
TATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGC399830.14246300916237786No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT399590.1423774950133671No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT397290.1415579844186807No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC384620.1370435499688212No Hit
AAATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTAT372200.13261819275751455No Hit
TTCGCTGGGTAGCTATGTGCGGACGGGCTGTAGGCACCATCAATATCTCG371250.13227969925101363No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT368140.13117157840341592No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA362240.12906935557356816No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATATCTCGTA352350.1255454600164166No Hit
AGCGGACAGATGGGAGCTTGCTCCCTGATGCTGTAGGCACCATCAATATC349470.12451929022828749No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA342750.12212489405598634No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC342690.12210351551873364No Hit
ATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTATGC337520.12026139822546027No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC332900.1186152508570032No Hit
AAAATTCCGTCGGAATCCGGGAGGACCCTGTAGGCACCATCAATATCTCG332040.11830882515638133No Hit
TAACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATATCTCGTATGCCGT325850.11610327272981223No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT320900.11433954340646539Illumina PCR Primer Index 6 (95% over 21bp)
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC317810.1132385487379519No Hit
TTAGGGCTAGCCTCAAGGTAAGAGTCTCTGTAGGCACCATCAATATCTCG315910.11256156172495008No Hit
CTGACTTATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTC313860.11183112836881655No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGCTGTAGGCACCATCAATATC298580.10638672754846505No Hit
TTGAGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTA285420.10169770171104192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)