FastQCFastQC Report
Wed 6 Sept 2023
SRR13142698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26335038
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2905601.1033209824872856Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT2044620.7763877158635579No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT1781300.6763992518256476No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1758030.6675631149649376No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1659090.6299933951111064No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC1374650.521985197059522No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC1283090.48721782744342346No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1190080.4518998605583937No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC918890.34892298237807745No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA894290.33958181491896844No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC868910.3299444641013998No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA788910.2995666837465737No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC731190.27764911522056657No Hit
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT653910.24830417939780458RNA PCR Primer, Index 6 (95% over 22bp)
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC625140.23737957013770022No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT604080.22938261945929223Illumina PCR Primer Index 6 (95% over 21bp)
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC589950.22401714400412104No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC579940.22021612423722345RNA PCR Primer, Index 6 (95% over 23bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCA570580.21666192393570877No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT565480.2147253404380886No Hit
AGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCATCA559450.2124356152438436No Hit
ACCGGGATGGACGCACCGCTGGTGTACCAGTCTGTAGGCACCATCAATAT527760.20040221700078809No Hit
AGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCTCG514870.19550759714111673No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC513640.19504053876816127No Hit
TTAGCGAAGTTCCTGATTCCACACTGCCACTGTAGGCACCATCAATATCT507780.19281536635717025No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC499550.18969025220316751Illumina PCR Primer Index 6 (95% over 23bp)
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT478200.18158318207097326No Hit
TGCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATAT475660.18061868754470756No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC466310.17706828446573725No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCA456320.17327485914392832No Hit
TGAGCCATTAGCTCAGTTGGTAGAGCATCTGCTGTAGGCACCATCAATAT430380.16342486386387595No Hit
AAGAACCTTACCAGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT426870.16209203875080797Illumina PCR Primer Index 6 (95% over 21bp)
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA364510.13841255896422097No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCACCATC358440.13610764487979854No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAACTGTAGGCA353750.13432674750649687No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC353540.13424700583306545No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATATCTCGTA350650.13314960851774735No Hit
AAAATTCCGTCGGAATCCGGGAGGACTGTAGGCACCATCAATATCTCGTA340210.12918530818144253No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGACTGTAGGCAC338990.12872204703103143No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT335570.12742339692086263Illumina PCR Primer Index 6 (95% over 22bp)
TCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGCCG325550.12361857993142066No Hit
TTGACTTATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTC318370.120892174144575No Hit
AGAAGGCAGACCCGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC313430.11901634620766449No Hit
TTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATCA312850.11879610730009199No Hit
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC295090.11205224006132058No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA285620.10845627031181805No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT281740.10698294796460897No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGCTGTAGGCAC279240.10603364232852067No Hit
GGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT275320.10454513109113418Illumina PCR Primer Index 6 (95% over 24bp)
TGGGGACGTTACGGATTCGACAGGCTGTAGGCACCATCAATATCTCGTAT269190.10221743367144563No Hit
TATCCTTGGGAGTCAGACTGCGCTGTAGGCACCATCAATATCTCGTATGC268490.10195162809334089No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT268120.10181113085919982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)