FastQCFastQC Report
Wed 6 Sept 2023
SRR13142699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57786912
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC1677750.29033390813476934No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1464720.2534691592449169Illumina PCR Primer Index 6 (96% over 29bp)
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC1171820.20278294157680551No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC883330.15285987249154273No Hit
ACGATGAGTGCTAAGTGTTAGGGGGCTGTAGGCACCATCAATATCTCGTA732180.12670343070070952No Hit
ATGCCTAAGGTTTCCTGAGGCTGTAGGCACCATCAATATCTCGTATGCCG606440.10494417836343288No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT579620.1003029890228431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)