FastQCFastQC Report
Wed 6 Sept 2023
SRR13142700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684637
Total Bases234.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT731871.562276863714307No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT655421.3990838564439465No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA558251.1916611681972369Illumina PCR Primer Index 6 (96% over 29bp)
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA401100.8562029459272937No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT251600.5370746975699505No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA178100.3801788697822265No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG168830.36039078374695843No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC150380.3210067290165705No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA123080.26273113583827307Illumina PCR Primer Index 6 (100% over 28bp)
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT121110.2585259007261395No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC111700.23843896549508534No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA104570.2232190028811197No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG95830.20456227451561346No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT90320.19280042402431607TruSeq Adapter, Index 6 (95% over 22bp)
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT89330.19068713328268552No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC85980.1835360989549457No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT77690.16583995729018067No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC76810.16396147663095348No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT72660.15510273261300714No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT68640.1465214914197194Illumina PCR Primer Index 6 (95% over 21bp)
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT67090.14321280389494426No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA62830.13411924979459455No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG57400.1225281702723178No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG52550.11217518027544077No Hit
TAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTC51720.1104034314718515No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA50970.10880245363728289No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC49560.1057926153082939No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC48920.10442644755612868No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC47950.10235584955675327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)