FastQCFastQC Report
Wed 6 Sept 2023
SRR13142701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13142701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106005236
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT14723921.3889804462111663No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT13462211.269957080233282No Hit
ATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCACC10083860.9512605584878845No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA8416550.7939749315779081No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6889280.6498999728654913Illumina PCR Primer Index 6 (96% over 29bp)
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC4835190.456127469024266No Hit
TATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG4785870.45147486865648784No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA4663350.439916949008066No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT4360630.4113598690540154No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT4198070.39602477749306647No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC3991760.3765625313074158No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT3871870.3652527126112903No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA3861580.3642820058435604No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT3301310.3114289562074085Illumina PCR Primer Index 6 (95% over 21bp)
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC2830880.26705095963372977No Hit
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC2615720.2467538490268537No Hit
TAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCA2472450.23323847889928756No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT2123540.20032406701118044No Hit
CGGAGTTACAAAGGAACTGTAGGCACCATCAATATCTCGTATGCCGTCTT1896140.17887229645901642TruSeq Adapter, Index 6 (95% over 22bp)
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG1858090.17528285112256153No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC1833940.17300466176972618No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC1828380.17248015937627836No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT1824780.1721405535100172No Hit
TTAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1807190.17048120151347995No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1800800.16987840110086636No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA1788710.16873789140000595No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1730200.1632183527236334Illumina PCR Primer Index 6 (100% over 28bp)
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG1723710.16260611881473477No Hit
AGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCAC1639950.1547046223263915No Hit
TATCAGTAGGTTGGGGGTTCAAGTCCTCTTGCCGGCACCACTGTAGGCAC1634410.1541820066322007No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1550430.14625975645203035No Hit
CATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCG1396260.13171613522939565No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1388590.13099258606433364No Hit
ATCAGAGGGTCGAAGGTTCGAGTCCTTCATGGCTCACCACTGTAGGCACC1305790.12318165114032668No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTACTGTAGG1300820.12271280637496057No Hit
TAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1231560.11617916684794702No Hit
AGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGCTGTAGGC1124560.10608532582296218No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1117110.10538253034972725No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGGTCCTGTAGGCACCATCA1112730.10496934321244282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)