FastQCFastQC Report
Wed 6 Sept 2023
SRR13150122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149647
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA162407611.47785524260782No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG8995886.357670972286446No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT8333175.889312998409077No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA4814953.4028764109804293No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3532862.496783135296591No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2861822.0225380887593873No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2078341.4688281622856034No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1845581.304329358887893No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1807511.2774240940427701No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA990260.6998478477943655No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT951010.6721086398833837No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT898370.6349062983691396No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG890230.6291535046775372No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG857210.6058172334617252No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA710650.5022386777564133No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC701340.4956590083130695No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA666990.4713827843196371No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA554750.39205925066540526No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT470870.33277861984825485No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA448930.31727293267457485No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA442920.31302547688998883No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA441900.31230461085000916No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG438070.3095978295430268No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG422340.2984809444362817No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG417020.2947211333257996No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT392500.2773920791098181No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT390370.27588674120280177No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCG388020.27422592238520155No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC369290.26098884304322223No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA338120.23896002493913807No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA337710.2386702650603227No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC329060.2325570383487305No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG320060.22619645564302768No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA307560.2173623129962182No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC307060.2170089472903458No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCCTGTAGGCACCATCA286910.20276830934368895No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGA272830.1928175310663227No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC256370.18118473202900398No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC237450.16781337371879312No Hit
TTTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA227550.16081673274252No Hit
TAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC226940.1603856265813557No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG212270.15001787677106007No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG204890.14480219895238375No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA202780.1433109956736023No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG185310.13096439791042136No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA178190.12593247025879867No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG174770.12351544883063159No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG167110.11810188621666676No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG165160.11672375996376447No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC161550.11417245956736588No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG153960.10880836815222315No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG153190.10826418496517969No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG152110.10750091504049535No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC146630.10362802690413407No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA143060.10110499576420529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)