FastQCFastQC Report
Wed 6 Sept 2023
SRR13150123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13150123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13604357
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length79
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA149554010.993095814818737No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG10446577.678841418230939No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT7957165.848979117498901No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7483945.501134673252106No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2432741.788206528246796No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA2300181.6907671564337807No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1852421.3616373048722552No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1730711.2721733191800244No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1303260.9579725083662536No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1230570.9045410966501393No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1219060.8960805718344498No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT830830.6107087604360868No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA813490.5979628438154041No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC630720.4636161782581859No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA624070.458728038377705No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT619440.4553247169270845No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA586920.43142061032358975No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC523170.38456062274755065No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC510130.3749754582300362No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG499500.3671617850075531No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG460630.3385900561121705No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA429670.31583264096936003No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA417440.306842873941047No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC396950.2917815226401365No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG386340.28398255059022637No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG375990.27637469378376356No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG349280.256741277812689No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA348650.25627819087664344No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC331200.24345141780680998No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG328550.24150351244090407No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA281580.20697780865350712No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA271070.19925234246646129No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG240510.1767889507751083No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA219390.1612645125381523No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA211090.1551635259204092No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCCTGTAGGCACCATCAATAGA202700.1489963840260881No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT200480.147364553870499No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA197060.14485065336053737No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG196950.14476979691138656No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG191300.14061671565954936No Hit
AAGAGGTACACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG176130.12946587626302367No Hit
TTTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA175060.12867936353037487No Hit
CCTGATTCAAGCTCAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA168000.12348984961215No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA158560.11655089615775298No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT155150.11404434623407779No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA154290.1134121958134442No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT151940.11168480803613137No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG146170.10744351974885692No Hit
GGGAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC144110.10592929897385081No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA140710.10343009963646205No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCCTGTAGGCACCATCA139960.10287880566497924No Hit
AAGAGGTGCACAATCGACCGATCCTACTGTAGGCACCATCAATAGATCGG139790.1027538456981098No Hit
AAGAGGTGTACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG136580.10039430750016336No Hit

[FAIL]Adapter Content

Adapter graph